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2.5.1.66: N2-(2-carboxyethyl)arginine synthase

This is an abbreviated version!
For detailed information about N2-(2-carboxyethyl)arginine synthase, go to the full flat file.

Word Map on EC 2.5.1.66

Reaction

D-glyceraldehyde 3-phosphate
+
L-arginine
=
N2-(2-carboxyethyl)-L-arginine
+
phosphate

Synonyms

carboxyethylarginine synthase, CEA synthetase, CEAS, CeaS1, ceaS2, N(2)-(2-carboxyethyl)arginine synthase, N2-(2-carboxyethyl)arginine synthetase

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.66 N2-(2-carboxyethyl)arginine synthase

Cloned

Cloned on EC 2.5.1.66 - N2-(2-carboxyethyl)arginine synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cea S gene of the gene cluster for clavulanic acid biosynthesis, genomic organization
-
ceas gene, expression in Escherichia coli BL21(DE3), first gene in the clavulanic acid cluster, orf2
-
ceaS1 and ceaS2, ceaS2 is located in the clavulanic acid gene cluster, ceaS1 in the paralogue gene cluster, study of the transcriptional regulation of the genes
-
Streptomyces venezuelae YJ028 is utilized as the heterologous host for the expression of four structural clavulanic acid biosynthesis genes, which encode carboxyethylarginine synthase (ceas2), beta-lactam synthetase (bls2), clavaminate synthase (cas2), and proclavaminate amidinohydrolase (pah2). The genes are cloned into pIBR25 expression vector containing ermE promoter to generate pBS4. The cas2 gene is also cloned into pSET152 to generate pCas2. It is then integrated into the genome of Streptomyces venezuelae YJ028