2.5.1.70: naringenin 8-dimethylallyltransferase
This is an abbreviated version!
For detailed information about naringenin 8-dimethylallyltransferase, go to the full flat file.
Word Map on EC 2.5.1.70
-
2.5.1.70
-
prenylated
-
sophora
-
flavescens
-
flavanones
-
flavonoids
-
isoprenoid
-
leachianone
-
2'-hydroxy
-
lavandulyl
-
sophoraflavanone
- 2.5.1.70
-
prenylated
-
sophora
- flavescens
- flavanones
- flavonoids
-
isoprenoid
-
leachianone
-
2'-hydroxy
-
lavandulyl
-
sophoraflavanone
Reaction
Synonyms
flavanone 8-dimethylallyltransferase, N8DT, N8DT-1, N8DT-3, N8DT1, N8DT2, naringenin 8-dimethylallyltransferase/chalcone isomerase, naringenin 8-prenyltransferase
ECTree
Advanced search results
Engineering
Engineering on EC 2.5.1.70 - naringenin 8-dimethylallyltransferase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
additional information
construction of chimeric enzymes between narigenin 8-dimethylallyltransferase and isoflavone-specific prenyltransferase G6DT responsible for the prenylation of the genistein at the 6-position, by recombination of amino acid sequences within the loop domains. Chimera SfG2N possesses purely narigenin 8-dimethylallyltransferase activity despite the absence of the N-terminal sequence derived from narigenin 8-dimethylallyltransferase, 243 amino acids. Chimera SfG3N possesses only isoflavone-specific prenyltransferase G6DT activity, although its 33% of the C-terminus is from narigenin 8-dimethylallyltransferase polypeptide. Neither chimeric enzyme can catalyze the prenylation reaction for other types of flavonoid such as kaempferol, apigenin, naringenin chalcone
additional information
-
construction of chimeric enzymes between narigenin 8-dimethylallyltransferase and isoflavone-specific prenyltransferase G6DT responsible for the prenylation of the genistein at the 6-position, by recombination of amino acid sequences within the loop domains. Chimera SfG2N possesses purely narigenin 8-dimethylallyltransferase activity despite the absence of the N-terminal sequence derived from narigenin 8-dimethylallyltransferase, 243 amino acids. Chimera SfG3N possesses only isoflavone-specific prenyltransferase G6DT activity, although its 33% of the C-terminus is from narigenin 8-dimethylallyltransferase polypeptide. Neither chimeric enzyme can catalyze the prenylation reaction for other types of flavonoid such as kaempferol, apigenin, naringenin chalcone
additional information
production of prenylated flavonoids in Solanum lycopersicum as a host plant by means of metabolic engineering through recombinant overexpression of both the naringenin 8-dimethylallyltransferase (N8DT) gene, encoding a prenyltransferase from Sophora flavescens, and the chalcone isomerase (CHI) gene from Nicotiana tabacum cv. Samsun-NN, in transgenic tomato lines. Production of 8-dimethylallyl naringenin in the double transformants, while the production level is lower than N8DT single transformants. Tomato fruits overexpressing both N8DT and CHI genes accumulate high levels of rutin compared with wild-type tomato
additional information
production of prenylated flavonoids in Solanum lycopersicum as a host plant by means of metabolic engineering through recombinant overexpression of both the naringenin 8-dimethylallyltransferase (N8DT) gene, encoding a prenyltransferase from Sophora flavescens, and the chalcone isomerase (CHI) gene from Nicotiana tabacum cv. Samsun-NN, in transgenic tomato lines. Production of 8-dimethylallyl naringenin in the double transformants, while the production level is lower than N8DT single transformants. Tomato fruits overexpressing both N8DT and CHI genes accumulate high levels of rutin compared with wild-type tomato