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2.5.1.73: O-phospho-L-seryl-tRNA:Cys-tRNA synthase

This is an abbreviated version!
For detailed information about O-phospho-L-seryl-tRNA:Cys-tRNA synthase, go to the full flat file.

Word Map on EC 2.5.1.73

Reaction

O-phospho-L-seryl-tRNACys
+
Sulfide
=
L-Cysteinyl-tRNACys
+
phosphate

Synonyms

MJ1678, Sep-tRNA-Cys-tRNA synthase, Sep-tRNA:Cys-tRNA synthase, SepCysS

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.73 O-phospho-L-seryl-tRNA:Cys-tRNA synthase

Purification

Purification on EC 2.5.1.73 - O-phospho-L-seryl-tRNA:Cys-tRNA synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DEAE column chromatography and Superdex 200 gel filtration
native enzyme to homogeneity
-
Q Sepharose FF column chromatography and FPLC Mono Q column chromatography
recombinant SepCysS1 from Escherichia coli strain BL21(DE3) by anion exchange chromatography and affinity chromatography on a heparin resin, recombinant selenomethionine-labeled SepCysS1 from Escherichia coli strain B834