2.5.1.75: tRNA dimethylallyltransferase

This is an abbreviated version!
For detailed information about tRNA dimethylallyltransferase, go to the full flat file.

Reaction

Synonyms

A37 isopentenyltransferase, alr5266, DELTA2-isopentenyl pyrophosphate:transfer ribonucleic acid DELTA2-isopentenyltransferase, DELTA2-isopentenyl pyrophosphate:tRNA-DELTA2-isopentenyl transferase, dimethylallyl (DELTA2-isopentenyl)diphosphate-tRNA transferase, dimethylallyl(D2-isopentenyl) diphosphate:tRNA transferase, dimethylallyl:tRNA[Ser]Sec transferase, DMAPP:tRNA[Ser]Sec transferase, DMATase, EC 2.5.1.8, IPT, IPT2, IPT9, isopentenyl transferase, isopentenyltransferase, isopentenyltransferase, transfer ribonucleate, MhIPT3, MiaA, Mod5, NoIPT2, prenyltransferase, Tit1, transfer ribonucleate isopentenyltransferase, TRIT1, tRNA IPT, tRNA isopentenyltransferase, tRNA([Ser]Sec)-isopentenyl transferase, tRNA-IPT, tRNA-IPT1

ECTree

     2 Transferases

         2.5 Transferring alkyl or aryl groups, other than methyl groups

             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

                2.5.1.75 tRNA dimethylallyltransferase

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Results	in table
43495	AA Sequence
4	Application
1	CAS Registry Number
11	Cloned(Commentary)
2	Crystallization (Commentary)
202	Disease/ Diagnostics
1	Expression
9	General Information
1	General Stability
11	Inhibitors
42	KM Value [mM]
4	Localization
13	Metals/Ions
6	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
34	Organism
4	Pathway
7	pH Optimum
2	pH Range
43	Protein Variants
9	Purification (Commentary)
5	Reaction
1	Reaction Type
34	Reference
12	Source Tissue
5	Specific Activity [micromol/min/mg]
5	Storage Stability
59	Substrates and Products (Substrate)
2	Subunits
41	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
19	Turnover Number [1/s]

Cloned

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Cloned on EC 2.5.1.75 - tRNA dimethylallyltransferase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

3 entries

expressed in Nicotiana tabacum cultivar Wisconsin 38

Homo sapiens

Q9H3H1

686911

expressed in Petunia hybrida under control of the flowerspecific Arabidopsis AP3 promoter

Agrobacterium tumefaciens

-

706146

expressed in Saccharomyces cerevisiae strain MT-8

Physcomitrium patens

A4ZZA3

676732

expressed in transgenic Nicotiana tabacum cv. Wisconsin 38, transcripts are found in leaves and stem but not in root

Agrobacterium tumefaciens

-

706187

expression in Escherichia coli

Nostoc sp. PCC 7120 = FACHB-418

Q8YLN2

759655

gene miaA, DNA and amino acid sequence determination and analysis, recombinant expression of the enzyme as maltose-binding-protein fusion protein in Escherichia coli strain BL21(DE3)

Salmonella enterica

P37724

737708

overexpressed as a N-terminal hexahistidine tag protein in Escherichia coli BL21 (DE3)

Humulus lupulus

Q5GHF7

705984

recombinant expression of His-tagged enzyme in Escherichia coli, recombinant overexpression of enzyme TRIT1 NIH 3T3 cells, recombinant overexpression of TRIT1 in HepG2 cells

Mus musculus

Q80UN9

737635

-

Escherichia coli

-

696190

-

Homo sapiens

Q9H3H1

489967

-

Arabidopsis thaliana

Q9ZUX7

489963