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2.5.1.80: 7-dimethylallyltryptophan synthase

This is an abbreviated version!
For detailed information about 7-dimethylallyltryptophan synthase, go to the full flat file.

Word Map on EC 2.5.1.80

Reaction

dimethylallyl diphosphate
+
L-tryptophan
=
diphosphate
+
7-(3-methylbut-2-enyl)-L-tryptophan

Synonyms

7-dimethylallyl tryptophan synthase, 7-dimethylallyltryptophan synthase, 7-DMATS, 7-DMATSNeo, ANI_1_660114, CAK41583, dimethylallyltryptophan synthase, tryptophan C7-prenyltransferase

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.80 7-dimethylallyltryptophan synthase

Purification

Purification on EC 2.5.1.80 - 7-dimethylallyltryptophan synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged 7-DMATS from Escherichia coli strains XL1 Blue or M15 or from Saccharomyces cerevisiae strain INVSc1 by nickel affinity chromatography
recombinant His6-tagged enzyme from Escherichia coli strain BL21 to near homogeneity by nickel affinity chromatography and desalting gel filtration
recombinant His6-tagged enzyme from Escherichia coli strain XL-1 Blue MRF' by nickel affinity chromatography to homogeneity
-
recombinant protein