2.5.1.82: hexaprenyl diphosphate synthase [geranylgeranyl-diphosphate specific]
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Reaction
+ 2 isopentenyl diphosphate = 2 diphosphate +
Synonyms
(all-E) hexaprenyl diphosphate synthase, (all-trans) hexaprenyl diphosphate synthase, EC 2.5.1.33, hexaprenyl pyrophosphate synthase, HexPPs, HexPS
ECTree
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Crystallization
Crystallization on EC 2.5.1.82 - hexaprenyl diphosphate synthase [geranylgeranyl-diphosphate specific]
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D81C and W136E mutant crystals are grown at room temperature by the hanging drop vapor diffusion method from a reservoir solution containing 10% (w/v) polyethyleneglycol 8000, 2.4 M lithium chloride, and 100 mM Tris (pH 8.0). Crystal structure of the homodimeric C30-HexPPs resembles those of other trans-prenyltransferases, including farnesyl pyrophosphate synthase (FPPs) and octaprenyl pyrophosphate synthase