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2.5.1.87: ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific]

This is an abbreviated version!
For detailed information about ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific], go to the full flat file.

Word Map on EC 2.5.1.87

Reaction

(2E,6E)-farnesyl diphosphate
+ n isopentenyl diphosphate = n diphosphate +
ditrans,polycis-polyprenyl diphosphate (n: 10-55)

Synonyms

AcpT, anther-66, cis-prenyltransferase, cis-PTase, CPT1, Dedol-PP synthase, DedolPP synthase, dehydrodolichyl diphosphate synthase, dehydrodolichyl diphosphate synthase 1, DHDDS, NgBR, Nogo-B receptor, RER2, Rer2p, Rer2p Z-prenyltransferase, SlCPT3, SRT1, Srt1p, Srt2p Z-prenyltransferase, Tk-IdsB

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.87 ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific]

Cloned

Cloned on EC 2.5.1.87 - ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific]

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Nicotiana benthamiana leaves
expression in Saccharomyces cerevisiae
expression in yeast strain SNH23-7D, the identity of the cloned enzyme is confirmed by functional complementation of a yeast mutant strain defective in dehydrodolichyl-diphosphates synthase activity
expression the gene in a yeast mutant strain SNH23-7D
functional expression in Saccaromyces cerevisiae nus1DELTA/rer2DELTA/srt1DELTA mutant strain
gene nus1, recombinant expression in the Saccharomyces cerevisiae triple deletion strain, nus1DELTA/rer2DELTA/srt1DELTA, functional complementation by recombinant expression of active Schizosaccharomyces pombe enzyme
gene rer2, DNA and amino acid sequence determination and analysis, recombinant expression in Saccharomyces cerevisiae strain KG219, expression of CaRER2 under control of the MET3 promoter in mutant strain JOS14
gene rer2, phylogenetic analysis, quantitative RT-PCR expression analysis, functional complementation of the yeast rer2DELTA dolichol mutant, production of dolichols in rer2DELTA strains expressing both SlCPT3 and SlCPTBP associating together in an enzyme complex, recombinant coexpression of C-terminally Myc-tagged SlCPT3 and FLAG-tagged SlCPTBP in Nicotiana benthamiana leaves using Agrobacterium tumefaciens strain LBA4404, recombinant expression of C-terminally Myc-tagged SlCPT3 in Escherichia coli
over-expression of SRT1 suppresses the growth and glycosylation defects of rer2
overexpression in Escherichia coli
when GFP-RER2 is over-expressed under the TDH3 promoter in DELTArer2, a continuous endioplasmic reticulum pattern and some dots associated with the endoplasmic reticulum are observed in the early logarithmic phase