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2.5.1.93: 4-hydroxybenzoate geranyltransferase

This is an abbreviated version!
For detailed information about 4-hydroxybenzoate geranyltransferase, go to the full flat file.

Word Map on EC 2.5.1.93

Reaction

geranyl diphosphate
+
4-hydroxybenzoate
=
3-geranyl-4-hydroxybenzoate
 +
diphosphate

Synonyms

4HB geranyltransferase, 4HB:geranyltransferase, AePGT, AePGT4, AePGT6, diphosphate:4-hydroxybenzoate geranyltransferase, geranyl diphosphate:4-hydroxybenzoate 3-geranyltransferase, geranyl diphosphate:4-hydroxybenzoate geranyltransferase, lepgt, LePGT-1, LePGT-2, LePGT1, p-hydroxybenzoate geranyltransferase, p-hydroxybenzoate-m-geranyltransferase, p-hydroxybenzoate: geranyltransferase, p-hydroxybenzoate:geranyltransferase, PGT, PGT-1, PHB geranyltransferase, PHBA geranyltransferase

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.93 4-hydroxybenzoate geranyltransferase

Cloned

Cloned on EC 2.5.1.93 - 4-hydroxybenzoate geranyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
all point mutants and chimeric enzymes are constitutively expressed in Saccharomyces cerevisiae containing a disrupted copy of the COQ2 gene
expression in Saccharomyces cerevisiae WAT11U strain and in a high yield monoterpene strain (HMT1). AePGT6 shows higher geranyltransferase activity in yeas than AePGT4 and AePGT
functional expression of LePGTs in a yeast COQ2 disruptant
PGT expression analysis
-
recombinant expression in Escherichia coli coenzyme Q8 biosynthesis-deficient strains, HW108 and HW109, which contain mutations in ubiE and ubiG, respectively. Gene lePGT-1 gene is amplified and cloned into the expression vector, pBAD33, to form pBlePGT, the fine-tuning of the expression of lePGT-1 in the ubiquinone-deficient strains, HW108 and HW109, to control the electron transfer chain is investigated by adding different concentrations of the inducer arabinose at 0-20 mM under fully aerobic conditions
recombinant expression of C-terminally His-tagged LePGT1 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection method