2.5.1.B31: 5-dimethylallyltryptophan synthase

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For detailed information about 5-dimethylallyltryptophan synthase, go to the full flat file.

Word Map on EC 2.5.1.B31

2.5.1.B31

indole

synthesis

7-dmats

fgapt2

dmapp

actinomycete

chemoenzymatic

regioselectivity

coelicolor

dimethylallyltryptophans

regiospecific

geranyl

Reaction

Synonyms

5-DMATS, 5-DMATSSc, C5-prenyltransferase, dimethylallyl-tryptophan synthase, EAW08391, SCO7467, tryptophan C5-prenyltransferase

ECTree

2 Transferases

2.5 Transferring alkyl or aryl groups, other than methyl groups

2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

2.5.1.B31 5-dimethylallyltryptophan synthase

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Results	in table
2	AA Sequence
9	Application
9	Cloned(Commentary)
11	General Information
1	Inhibitors
16	kcat/KM [mM/s]
36	KM Value [mM]
12	Metals/Ions
20	Natural Substrates/ Products (Substrates)
17	Organism
11	pH Optimum
9	Purification (Commentary)
2	Reaction
4	Reaction Type
15	Reference
1	Source Tissue
115	Substrates and Products (Substrate)
25	Synonyms
1	Systematic Name
12	Temperature Optimum [°C]
1	Temperature Stability [°C]
30	Turnover Number [1/s]

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Cloned on EC 2.5.1.B31 - 5-dimethylallyltryptophan synthase

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5-DMATS, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strains BL21, XL1 Blue, or M15 or in Saccharomyces cerevisiae strain INVSc1

3 entries

gene ACLA_031240 or 5-dmats, DNA and amino acid sequence determination and analysis, expression as solube His-tagged protein in Escherichia coli strain M15, subcloning in Escherichia coli strain XL1 Blue

Aspergillus clavatus

A1CRX0

722750

gene SCO7467, recombinant expression of His8-tagged enzyme in Escherichia coli strain BL21(DE3)

Streptomyces coelicolor

Q9ADJ2

737406

gene SCO7468, genetic organization and sequence comparison, functional recombinant expression in Streptomyces lividans strain TK23, the overexpression of 5-DMATSSc results in 5-dimethylallylindole-3-acetonitrile overproduction in the transformant. The heterologous host Streptomyces lividans TK23 possesses a gene cluster almost identical to that of Streptomyces coelicolor A3(2). Recombinant expression in Escherichia coli strain BL21(DE3)

Streptomyces coelicolor

Q9ADJ2

738605

recombinant overexpression

3 entries