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2.5.1.B31: 5-dimethylallyltryptophan synthase

This is an abbreviated version!
For detailed information about 5-dimethylallyltryptophan synthase, go to the full flat file.

Word Map on EC 2.5.1.B31

Reaction

dimethylallyl diphosphate
+
L-tryptophan
=
diphosphate
+
5-(3-methylbut-2-enyl)-L-tryptophan

Synonyms

5-DMATS, 5-DMATSSc, C5-prenyltransferase, dimethylallyl-tryptophan synthase, EAW08391, SCO7467, tryptophan C5-prenyltransferase

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.B31 5-dimethylallyltryptophan synthase

Purification

Purification on EC 2.5.1.B31 - 5-dimethylallyltryptophan synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
recombinant enzyme from in Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant His-tagged 5-DMATS from Escherichia coli strain BL21 or Saccharomyces cerevisiae strain INVSc1 by nickel affinity chromatography
recombinant His8-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant solube His-tagged 5-DMATS from Escherichia coli strain M15 by nickel affinity chromatography to near homogeneity