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2.6.1.1: aspartate transaminase

This is an abbreviated version!
For detailed information about aspartate transaminase, go to the full flat file.

Word Map on EC 2.6.1.1

Reaction

L-aspartate
+
2-oxoglutarate
=
oxaloacetate
 +
L-glutamate

Synonyms

2-oxoglutarate-glutamate aminotransferase, AAT, AAT-2, AAT-3, AAT3, aatA, AATase, aatB3, all2340, alr1039, alr2765, alr4853, aminotransferase, aspartate, aoa/coa, AsAT, Asp AT, aspartate alpha-ketoglutarate transaminase, aspartate aminotransferase, aspartate aminotransferase 1, aspartate aminotransferase A, aspartate AT, aspartate transaminase, aspartate, 2-oxoglutarate aminotransferase, aspartate-2-oxoglutarate transaminase, aspartate/(R)-cysteate:2-oxoglutarate aminotransferase, aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase, aspartate:2-oxoglutarate amino-transferase, aspartate:2-oxoglutarate aminotransferase, aspartic acid aminotransferase, aspartic aminotransferase, aspartyl aminotransferase, AspAT, AspATSs, aspB, aspC, AspT, AST, AST-Bb, AtPAT, bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase, C-S lyase, CAA1, cAST, Cgl0240, class Ibeta AAT, EcAspAT, GL50803_91056, glutamate oxaloacetate transaminase, glutamate oxaloacetate transaminase 1, glutamate-oxalacetate aminotransferase, glutamate-oxalate transaminase, glutamate-oxaloacetate transaminase 1, glutamic oxalic transaminase, glutamic oxaloacetic transaminase, glutamic-aspartic aminotransferase, glutamic-aspartic transaminase, glutamic-oxalacetic transaminase, glutamic-oxaloacetic transaminase, GOT, GOT (enzyme), GOT1, GOT1L1, GOT2, KAT IV, L-aspartate aminotransferase, L-aspartate transaminase, L-aspartate-2-ketoglutarate aminotransferase, L-aspartate-2-oxoglutarate aminotransferase, L-aspartate-2-oxoglutarate-transaminase, L-aspartate-alpha-ketoglutarate transaminase, L-aspartate:2-oxoglutarate aminotransferase, L-aspartateartate aminotransferase, L-aspartic aminotransferase, L-AspAT, mitAAT, More, oxaloacetate transferase, oxaloacetate-aspartate aminotransferase, Pat, PfAspAT, plastid aspartate aminotransferase, prephenate aminotransferase, protein TT0402, PT-AAT, Rv3722c, Sar2028, SsAspAT, Tb11.02.2740, transaminase A

ECTree

     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.1 aspartate transaminase

Cloned

Cloned on EC 2.6.1.1 - aspartate transaminase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AAT1, AAT2, and AAT5, overexpression in Escherichia coli strains
-
aspartate aminotransferase construct consists of the Arabidopsis thaliana ASP5 coding region followed by mGFP5. This construct is designated SP5:GFP. GFP is fused to the carboxy-terminus of ASP5 to preserve the cleavage site between the ASP5 transit peptide and mature protein, thus ensuring proper plastid import and processing. A five amino acid linker peptide (GSGGG) is inserted between ASP5 and GFP to promote proper folding and activity of both proteins. The constructs are introduced into Nicotiana tabacum cv. Samsun NN via Agrobacterium-mediated transformation of leaf strips. ASP5:GFP is present in stromules of Nicotiana tabacum. Arabidopsis thaliana ASP5, with GFP fused to its carboxy-terminus, can interact with itself and with the Nicotiana tabacum AAT3 to form enzymes that are functional in vitro
-
cloning and DNA-sequencing of cDNA encoding the plastidic isozyme pAspAT, DNA and amino acid sequence analysis
-
cloning of a liver cDNA fragment, nucleotides 1-419, into a plasmid for production of cRNA probes
-
cloning, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli AB1157
-
expressed in Escherichia coli
expressed in Escherichia coli as a His-tagged fusion protein
expressed in Escherichia coli BL21 (DE3) cells
-
expressed in Escherichia coli BL21 CodonPlus (DE3)-RIL cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) Star cells
expressed in Escherichia coli One Shot BL21 (DE3) cells
expressed in Escherichia coli strain MG204
expressed in Escherichia coli T7 Express LacIQ cells
expression in Escherichia coli
expression in Escherichia coli, His-tagged protein
expression in Mycobacterium smegmatis
expression of wild-type mAspAT and pmAspAT and mutants in Escherichia coli
-
gene aspC, expression of wild-type and K109 mutants in Escherichia coli BL21(DE3)
isolation of genomic DNA and construction of a genetic library, cloning of gene PhaspC, DNA and amino acid sequence determination, overexpression in Escherichia coli strain TY103
-
overexpressed as His6-tagged protein in Escherichia coli cells. The production of the protein corresponding to the gene GOT1L1 encounters several problems: upon induction, multiple overexpressed protein species are detected in the cell lysate, and none of them could be purified by metal-affinity chromatography
overexpressed in Escherichia coli
-
overexpression from plasmid in strain JM103
-
overexpression of wild-type enzyme and mutants from plasmid in Escherichia coli strain MG204, which lacks endogenous enzyme activity
-