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2.6.1.1: aspartate transaminase

This is an abbreviated version!
For detailed information about aspartate transaminase, go to the full flat file.

Word Map on EC 2.6.1.1

Reaction

L-aspartate
+
2-oxoglutarate
=
oxaloacetate
 +
L-glutamate

Synonyms

2-oxoglutarate-glutamate aminotransferase, AAT, AAT-2, AAT-3, AAT3, aatA, AATase, aatB3, all2340, alr1039, alr2765, alr4853, aminotransferase, aspartate, aoa/coa, AsAT, Asp AT, aspartate alpha-ketoglutarate transaminase, aspartate aminotransferase, aspartate aminotransferase 1, aspartate aminotransferase A, aspartate AT, aspartate transaminase, aspartate, 2-oxoglutarate aminotransferase, aspartate-2-oxoglutarate transaminase, aspartate/(R)-cysteate:2-oxoglutarate aminotransferase, aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase, aspartate:2-oxoglutarate amino-transferase, aspartate:2-oxoglutarate aminotransferase, aspartic acid aminotransferase, aspartic aminotransferase, aspartyl aminotransferase, AspAT, AspATSs, aspB, aspC, AspT, AST, AST-Bb, AtPAT, bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase, C-S lyase, CAA1, cAST, Cgl0240, class Ibeta AAT, EcAspAT, GL50803_91056, glutamate oxaloacetate transaminase, glutamate oxaloacetate transaminase 1, glutamate-oxalacetate aminotransferase, glutamate-oxalate transaminase, glutamate-oxaloacetate transaminase 1, glutamic oxalic transaminase, glutamic oxaloacetic transaminase, glutamic-aspartic aminotransferase, glutamic-aspartic transaminase, glutamic-oxalacetic transaminase, glutamic-oxaloacetic transaminase, GOT, GOT (enzyme), GOT1, GOT1L1, GOT2, KAT IV, L-aspartate aminotransferase, L-aspartate transaminase, L-aspartate-2-ketoglutarate aminotransferase, L-aspartate-2-oxoglutarate aminotransferase, L-aspartate-2-oxoglutarate-transaminase, L-aspartate-alpha-ketoglutarate transaminase, L-aspartate:2-oxoglutarate aminotransferase, L-aspartateartate aminotransferase, L-aspartic aminotransferase, L-AspAT, mitAAT, More, oxaloacetate transferase, oxaloacetate-aspartate aminotransferase, Pat, PfAspAT, plastid aspartate aminotransferase, prephenate aminotransferase, protein TT0402, PT-AAT, Rv3722c, Sar2028, SsAspAT, Tb11.02.2740, transaminase A

ECTree

     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.1 aspartate transaminase

Purification

Purification on EC 2.6.1.1 - aspartate transaminase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
1 cytosolic isozyme, partial, 120fold
-
2 forms: A and B
Leptosphaeria michotii
-
2 isoenzymes: AAT-1, AAT-2
-
2 isozymes
200-1000fold, copurification with the aromatic amino acid aminotransferase 2.6.1.57
-
380fold
-
by anion-exchange chromatography
-
crystal structures of the internal aldimine and the stable L-aspartate external aldimine of Escherichia coli AAT reconstituted with deazaPLP at 1.8 A resolution is reported. Crystals are obtained by the hanging drop method
cytosolic and mitochondrial isozymes
dolphin
-
cytosolic form I, and mitochondrial forms II and III
-
cytosolic isozyme
cytosolic isozyme: multiple forms AAT-2a, AAT-2b, AAT-2c
-
DEAE-Sepharose column chromatography and Phenyl-Sepharose column chromatography
differential centrifugation, Sephadex G-25 gel filtration, DEAE cellulose ion exchange chromatography, and Sephadex G-150 gel filtration
-
EcAspAT is purified by anion exchange chromatography in a Fractogel EMD DEAE 650-S column, pooled fractions are dialyzed against 20 mM sodium phosphate, pH 7.5, and loaded onto a ceramic hydroxyapatite column
His SpinTrap column chromatography
His-tag affinity chromatography and gel filtration
HisTrap column chromatography and Sephacryl S-200 gel filtration
HiTrap QFF column chromatography, HiTrap SPFF column chromatography and Superdex 200X gel filtration
isoenzyme I and II
-
isoenzyme mitAAT DEAE-Sepharose column chromatography
-
isoenzymes A and B
-
isoenzymes: AAT-P1 and AAT-P2, partial
-
isoenzymes: Asp-DEAE-1 and Asp-DEAE-2
-
isoenzymes: AspAT-1, AspAT-2, AspAT-3
-
isozyme AST II, 30.25fold
-
isozymes AAT1 and AAT2
-
isozymes mAsp-AT, cAsp-AT, and pAspAT from young green leaves
-
large-scale
-
mitochondrial and cytosolic isozymes
mitochondrial isoform
Torulopsis candida
-
mitochondrial isozyme
mitochondrial isozyme, 57.3fold
-
mutant V39L
-
Ni-NTA affinity chromatography and DEAE Sephacel column chromatography
-
Ni-NTA column chromatography
Ni-NTA column chromatography and Sephacryl S300 gel filtration
Ni-NTA Superflow resin chromatography
nickel Sepharose 6 fast flow chromatography and S-75 gel filtration
-
partial
partial purification of native enzyme, purification of recombinant enzyme from Escherichia coli
-
partial, 404fold, cytosolic isozyme
-
partial, 92fold
-
partial, cytosolic, 171fold, and mitochondrial, 78fold, isozymes
-
recombinant from overproducing Escherichia coli AB1157 cells, 66fold
-
recombinant isozymes AAT1, AAT2 and AAT5 from Escherichia coli
-
recombinant mAspAT, pmAspAT and mutants from Escherichia coli
-
recombinant protein, rapid protocol for purification
-
recombinant wild-type and mutants from overexpressing strain MG204
-
recombinantly overexpressed enzyme
-
Sephadex G-25 gel filtration and DEAE-Toyopearl column chromatography
-
SP-Sepharose column chromatography
-
using Ni-NTA chromatography, anion-exchange chromatography and gel filtration
-