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2.6.1.21: D-amino-acid transaminase

This is an abbreviated version!
For detailed information about D-amino-acid transaminase, go to the full flat file.

Word Map on EC 2.6.1.21

Reaction

D-alanine
+
2-oxoglutarate
=
pyruvate
+
D-glutamate

Synonyms

aminotransferase, D-alanine, AspFum, AspOry, AspTer, AtDAT1, D-AA transaminase, D-AAT, D-alanine aminotransferase, D-alanine transaminase, D-alanine:2-oxoglutarate aminotransferase, D-amino acid aminotransferase, D-amino acid transaminase, D-aspartate transaminase, D-aspartic aminotransferase, DAA aminotransferase, DAA transaminase, DAAT, DAT, DatA, Dret, Dret_1107, EC 2.6.1.10, FQP89_01185, GibZea, MSMEG_5795, MycVan, NeoFis, PenChr, TRA-01185

ECTree

     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.21 D-amino-acid transaminase

Crystallization

Crystallization on EC 2.6.1.21 - D-amino-acid transaminase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystallized from solutions containing 0.05 mM pyridoxal 5'-phosphate, 1-2% dioxane, 5 mM sodium azide, 18-22% poly(ethylene glycol) 4000, and 25 mM 2-(N-morpholino)ethanesulfonic acid, pH 5.5-6.5 by the hanging drop method, 1.94 A resolution
-
pyridoxal 5'-phosphate and pyridoxamine 5'-phosphate form of L201A mutant enzyme, crystallization from solutions containing 100 mM Tris-HCl, pH 8.5-9.9, 29-33% polyethylenglycol 3350, 300-700 mM sodium acetate, and 5 mM sodium azide by the hanging drop method, crystal structures at 2.0 A resolution
-
pyridoxal 5'-phosphate form: hanging drop method, 25% polyethylene glycol 4000, 200 mM ammonium sulfate, 100 mM sodium acetate, pH 4.6 and 1 mM 2-oxoglutarate, single yellow crystals after 2 weeks, enzyme complexed with N-(5'-phosphopyridoxyl)-D-alanine: hanging drop method, 26-28% polyethylene glycol 4000, 300 mM sodium acetate, 100 mM Tris-chloride, pH 8.5, crystal structure at 2.4 and 1.9 A resolution, respectively