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2.6.1.42: branched-chain-amino-acid transaminase

This is an abbreviated version!
For detailed information about branched-chain-amino-acid transaminase, go to the full flat file.

Word Map on EC 2.6.1.42

Reaction

L-leucine
+
2-oxoglutarate
=
4-methyl-2-oxopentanoate
 +
L-glutamate

Synonyms

(R)-selective omega-aminotransferase, AGT, alpha-KGA, At1g50110, At5g17310, Bat1, Bat1p, BAT2, Bat2p, BCAA, BCAA aminotransferase, BCAA-AT, BCAATase, BcaT, BCAT-1, BCAT1, Bcat2, BCAT4, Bcat6, BCAT7, BCATc, BCATm, branched chain amino acid: 2-oxoglutarate aminotransferase EC 2.6.1, branched chain aminotransferase, branched chain aminotransferase IlvE, branched chain aminotransferase, cytosolic isoform, branched chain aminotransferase, mitochondrial isoform, branched-chain amino acid aminotransferase, branched-chain amino acid aminotransferase 1, branched-chain amino acid aminotransferase 2, branched-chain amino acid aminotransferase 3, branched-chain amino acid aminotransferase 4, branched-chain amino acid aminotransferase 5, branched-chain amino acid aminotransferase 6, branched-chain amino acid transaminase, branched-chain amino acid transaminase 1, branched-chain amino acid transferase, branched-chain amino acid-glutamate transaminase, branched-chain amino-acid aminotransferase, branched-chain amino-acid transaminase, branched-chain aminotransferase, branched-chain aminotransferase 7, branched-chain aminotransferase4, CsBCAT2, CsBCAT3, CsBCAT7, eBCAT, EC 2.6.1.6, glutamate-branched-chain amino acid transaminase, hBCAT, hBCATm, Hoch3033, IlvE, IlvE aminotransferase, ilvE1, L-branched chain amino acid aminotransferase, L-leucine-alpha-ketoglutarate transaminase, leucine aminotransferase, leucine transaminase, Mevan_0408, mitochondrial branched chain aminotransferase, More, MtIlvE, PsBCAT, R-omegaAT, R-omegaAT_Bcel, R-omegaAT_Bthu, Rv2210c, SlBCAT1, SlBCAT2, SlBCAT3, SlBCAT4, SlBCAT5, SlBCAT6, SpBCAT1, TA-B, transaminase B, trd_1610, Tter_1720, TUZN1299, VMUT0738, VMUT_0738

ECTree

     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.42 branched-chain-amino-acid transaminase

Purification

Purification on EC 2.6.1.42 - branched-chain-amino-acid transaminase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 isoenzymes, AT-I and AT-II
-
2 isoenzymes, partially
-
DEAE Toyopearl column chromatography, Phenyl Sepharose column chromatography, DEAE Sepharose column chromatography, Shodex KW-2003 gel filtration, and Butyl Toyopearl gel filtration
Glutathione-Superflow Resin
-
GST-affinity chromatography, cleavage of the GST tag, anion exchange chromatography, gel filtration
His-tag Ni2+-NTA column chromatography
-
hog heart, partial
-
immobilized metal ion affinity chromatography (Ni2+)
immobilized metal ion affinity chromatography (Ni2+), anion-exchange chromatography (removal of the His-tag after cleavage)
-
immobilized metal ion affinity chromatography (Ni2+), gel filtration
mitochondrial and cytosolic isoenzymes
-
native enzyme to homogeneity
Ni-NTA acid resin column chromatography
Ni-NTA resin column chromatography and Mono-Q HR 5/5 column chromatography
-
Ni2+ affinity column chromatography and S75 gel filtration
partial
partially
recombinant Bcatm and BCATc, expressed in E. coli
-
recombinant enzyme
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chrormatography
-
recombinant His-tagged isozyme BCATm (residues 28-392) from Escherichia coli strain BL21Star (DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration, followed by tag cleavage through thrombin, hydrophic interaction chromatography, and dialysis
recombinant His-tagged isozyme from Escherichia coli strain Rosetta(DE3) by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, anion exchange chromatography, and dialysis
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLys by nickel affinity chromatography, tag cleavage by TEV protease, and gel filtration, to homogeneity
recombinant His6-tagged enzyme from Escherichia coli strain DLT1270 by nickel affinity chromatography and gel filtration
recombinant N-terminally His-tagged BCAT2 by nickel affinity chromatography, tag cleavage by TEV protease, followed by gel filtration and ultrafiltration
recombinant protein
-
S-protein agarose chromatography
-
TALON Affinity Purification Resin
-
TALON Affinity Purification Resin or Glutathione-Superflow Resin
TALON Affinity Purification Resin, Glutathione-Superflow Resin
-