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20
3-hydroxykynurenine
in 100 mM potassium phosphate buffer (pH 7.5) for 5 min at 45°C
12
kynurenine
in 100 mM potassium phosphate buffer (pH 7.5) for 5 min at 45°C
0.33
glycine
pH 7.4, 25°C, wild-type enzyme
0.33
glycine
wild-type, 25°C
0.068
glyoxylate
mutant G82E, 25°C
0.068
glyoxylate
pH 7.4, 25°C, mutant enzyme G82E
0.068
glyoxylate
-
G82E, pH not specified in the publication, temperature not specified in the publication
11.1
glyoxylate
-
P11L/I340M/G41R, pH not specified in the publication, temperature not specified in the publication
16
glyoxylate
-
pH 7.0, 50°C, L-alanine as amino donor
18.3
glyoxylate
-
G41V, pH not specified in the publication, temperature not specified in the publication
19
glyoxylate
recombinant mutant R118A, pH 7.4, 25°C
20
glyoxylate
recombinant mutant F240S, pH 7.4, 25°C
20.5
glyoxylate
-
G41R, pH not specified in the publication, temperature not specified in the publication
22
glyoxylate
recombinant mutant I56N-Mi, pH 7.4, 25°C
22
glyoxylate
recombinant mutant P11R/I56N-Ma, pH 7.4, 25°C
22.6
glyoxylate
major allele wild mutant enzyme F152A
23
glyoxylate
recombinant mutant P11R-Ma, pH 7.4, 25°C
30
glyoxylate
recombinant mutant I56N-Ma, pH 7.4, 25°C
33
glyoxylate
recombinant wild-type enzyme AGT-Mi, pH 7.4, 25°C
35
glyoxylate
recombinant mutant F238S, pH 7.4, 25°C
37
glyoxylate
minor allele wild type enzyme
37
glyoxylate
-
P11L/I340M minor allele, pH not specified in the publication, temperature not specified in the publication
39
glyoxylate
major allele wild mutant enzyme F152I
39
glyoxylate
-
F152I, pH not specified in the publication, temperature not specified in the publication
39
glyoxylate
recombinant wild-type enzyme AGT-Mi, pH 7.4, 25°C
40
glyoxylate
minor allele mutant enzyme F152I
40
glyoxylate
-
P11L/I340M/F152I, pH not specified in the publication, temperature not specified in the publication
45
glyoxylate
pH 7.4, 25°C, wild-type enzyme
45
glyoxylate
wild-type, 25°C
45
glyoxylate
major allele wild type enzyme
45
glyoxylate
-
major allele (P11, I340), pH not specified in the publication, temperature not specified in the publication
45
glyoxylate
recombinant wild-type enzyme AGT-Ma, pH 7.4, 25°C
113
glyoxylate
pH 7.0, 50°C, L-alanine as amino donor
0.07
L-alanine
mutant G82E, 25°C
0.07
L-alanine
pH 7.4, 25°C, mutant enzyme G82E
0.07
L-alanine
-
G82E, pH not specified in the publication, temperature not specified in the publication
0.147
L-alanine
pH 7.4, 25°C
3 - 6
L-alanine
recombinant mutant F238S, pH 7.4, 25°C
10.6
L-alanine
-
P11L/I340M/G41R, pH not specified in the publication, temperature not specified in the publication
17.1
L-alanine
-
G41V, pH not specified in the publication, temperature not specified in the publication
18
L-alanine
recombinant mutant I56N-Mi, pH 7.4, 25°C
19
L-alanine
recombinant mutant P11R/I56N-Ma, pH 7.4, 25°C
19.8
L-alanine
-
G41R, pH not specified in the publication, temperature not specified in the publication
20
L-alanine
-
pH 7.0, 50°C, glyoxylate as amino acceptor
21
L-alanine
recombinant mutant R118A, pH 7.4, 25°C
21.2
L-alanine
major allele mutant enzyme F152A
22
L-alanine
recombinant mutant F240S, pH 7.4, 25°C
22
L-alanine
recombinant mutant P11R-Ma, pH 7.4, 25°C
29
L-alanine
recombinant mutant I56N-Ma, pH 7.4, 25°C
33
L-alanine
minor allele wild type enzyme
33
L-alanine
-
P11L/I340M minor allele, pH not specified in the publication, temperature not specified in the publication
33.6
L-alanine
minor allele mutant enzyme F152I
33.6
L-alanine
-
P11L/I340M/F152I, pH not specified in the publication, temperature not specified in the publication
34.2
L-alanine
in 100 mM potassium phosphate buffer (pH 7.5) for 5 min at 45°C
35
L-alanine
major allele mutant enzyme F152I
35
L-alanine
-
F152I, pH not specified in the publication, temperature not specified in the publication
35
L-alanine
recombinant wild-type enzyme AGT-Mi, pH 7.4, 25°C
37
L-alanine
recombinant wild-type enzyme AGT-Mi, pH 7.4, 25°C
45
L-alanine
pH 7.4, 25°C, wild-type enzyme
45
L-alanine
wild-type, 25°C
45
L-alanine
major allele wild type enzyme
45
L-alanine
-
major allele (P11, I340), pH not specified in the publication, temperature not specified in the publication
45
L-alanine
recombinant wild-type enzyme AGT-Ma, pH 7.4, 25°C
117
L-alanine
in 100 mM potassium phosphate buffer (pH 7.5) for 5 min at 45°C
131
L-alanine
pH 7.0, 50°C, glyoxylate as amino acceptor
0.36
pyruvate
pH 7.4, 25°C, wild-type enzyme
0.36
pyruvate
wild-type, 25°C