2.6.1.52: phosphoserine transaminase

This is an abbreviated version!
For detailed information about phosphoserine transaminase, go to the full flat file.

Reaction

(3R)-3-hydroxy-2-oxo-4-phosphooxybutanoate

Synonyms

3-O-phospho-L-serine:2-oxoglutarate aminotransferase, 3-phosphoserine aminotransferase, AspAT, AtPSAT, AtPSAT1, BCIR PSAT, bmPSAT, EhPSAT, hydroxypyruvic phosphate-glutamic transaminase, L-phosphoserine aminotransferase, phosphohydroxypyruvate transaminase, phosphohydroxypyruvic-glutamic transaminase, phosphoserine aminotransferase, phosphoserine aminotransferase 1, phosphoserine aminotransferase1, phosphoserine aminotransferase2, phosphoserine aminotransferases, PSAT, PSAT alpha, PSAT beta, PSAT-BALC, PSAT-BCIRA, PSAT-ECOLI, PSAT1, PSAT2, PSerAT, serC, sll1559

ECTree

2 Transferases

2.6 Transferring nitrogenous groups

2.6.1 Transaminases

2.6.1.52 phosphoserine transaminase

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Results	in table
34262	AA Sequence
7	Application
1	CAS Registry Number
23	Cloned(Commentary)
25	Cofactor
10	Crystallization (Commentary)
266	Disease/ Diagnostics
5	Expression
32	General Information
3	General Stability
1	IC50 Value
13	Inhibitors
4	kcat/KM [mM/s]
4	Ki Value [mM]
60	KM Value [mM]
10	Localization
31	Molecular Weight [Da]
28	Natural Substrates/ Products (Substrates)
65	Organism
14	Pathway
77	PDB ID
21	pH Optimum
10	pH Range
1	pH Stability
7	pI Value
35	Protein Variants
21	Purification (Commentary)
4	Reaction
1	Reaction Type
58	Reference
57	Source Tissue
43	Specific Activity [micromol/min/mg]
3	Storage Stability
74	Substrates and Products (Substrate)
21	Subunits
96	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
3	Temperature Stability [°C]
10	Turnover Number [1/s]

Purification

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Purification on EC 2.6.1.52 - phosphoserine transaminase

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7 entries

MalE/PdxC(SerC) fusion protein, affinity chromatography on an amylose resin, hydrolyzed in the presence of protease factor Xa

Escherichia coli

722253

native enzyme from rat brain by ultracentrifugation at 220000 x g, followed by ammonium sulfate fractionation, hydropbobic interaction chromatography, anion exchange chromatography, and hydroxyapatite chromatography

recombinant

recombinant enzyme

recombinant enzyme 15.6fold from Escherichia coli strain BL21(DE3) by two different steps of anion exchange chromatography, ultrafiltration, and gel filtration

Alkalihalobacillus alcalophilus

Q9RME2

759886

recombinant enzyme mutant K2G 5.5fold from Escherichia coli strain XL-1 Blue by anion exchange chromatography, ultrafiltration, and gel filtration

Niallia circulans

Q59196

759886

recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

Escherichia coli

P23721

758955

recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration. The N-terminal 45 amino acid residues of wild-type EhPSAT protein can be truncated either at the time of purification or in vivo

Entamoeba histolytica

Q60I38

759344

recombinant His6-tagged from Escherichia coli strain BL21 Gold by nickel affinity chromatography, tag cleavage through TEV protease, dialysis, gel filtration and ultrafiltration

Arabidopsis thaliana

Q96255

759284

recombinant SUMO-His6-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration, to homogeneity

Bombyx mori

H9JSH4, Q2F5M8

758704