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2.6.1.97: archaeosine synthase

This is an abbreviated version!
For detailed information about archaeosine synthase, go to the full flat file.

Word Map on EC 2.6.1.97

Reaction

L-glutamine
+
7-cyano-7-carbaguanine15 in tRNA
 +
H2O
=
L-glutamate
 +
archaeine15 in tRNA

Synonyms

ARChaeosine synthase, archaeosine synthase subunit alpha, ArcS, ATP-independent amidinotransferase, lysine transferase, MA_4632, MJ1022, QueF-L, QueF-like, SSO0016, Ta0924, TaArcS, TgtA2, TK2156, TkArcS, tRNA-guanine transglycosylase

ECTree

     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.97 archaeosine synthase

Cloned

Cloned on EC 2.6.1.97 - archaeosine synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
gene arcS, recombinant expression of enzyme ArcS in an arcS orthologue (KEGG accession TK2156)-deletion strain of Thermococcus kodakarensis and functional complementation
gene tgtA2
-
in the Escherichia coli K12 MG1655 strain, the queF and queC are deleted (DELTAque/DELTAqueF), and the resulted deletion strain is transformed with an expression plasmid containing GAT-queC from S. solfataricus (SSO0016) cloned behind a PBAD promoter
recombinant coexpression of His-tagged enzyme in Escherichia coli strain ER2566
recombinant coexpression of His-tagged TaArcS and His-tagged TkRaSEA in Escherichia coli