2.6.99.2: pyridoxine 5'-phosphate synthase

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For detailed information about pyridoxine 5'-phosphate synthase, go to the full flat file.

Reaction

Synonyms

PdxJ, PNP synthase, pyridoxine 5'-phosphate synthesizing enzyme

ECTree

     2 Transferases

         2.6 Transferring nitrogenous groups

             2.6.99 Transferring other nitrogenous groups

                2.6.99.2 pyridoxine 5'-phosphate synthase

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Results	in table
21707	AA Sequence
7	Application
1	CAS Registry Number
4	Crystallization (Commentary)
1	KM Value [mM]
13	Natural Substrates/ Products (Substrates)
15	Organism
5	Pathway
28	PDB ID
2	pH Optimum
2	Protein Variants
1	Purification (Commentary)
7	Reaction
15	Reference
28	Substrates and Products (Substrate)
3	Subunits
19	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]

Crystallization

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Crystallization on EC 2.6.99.2 - pyridoxine 5'-phosphate synthase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

12 mg/ml purified recombinant enzyme, wild-type or selenomethionine-labeled, in 10 mM Tris-HCl, pH 7.5, 0.4 mM potassium phosphate, 1.21 mM NaCl, 8% PEG 8000, 9% PEG 1000, 10% glycerol, and 4 mM 1-deoxy-D-xylulose, hanging drop vapour diffusion method, X-ray diffraction structure determination and analysis at 1.96-3.2 A resolution

Escherichia coli

P0A794

664004

6 mg/ml purified recombinant enzyme in 2 mM Tris-HCl, pH 8.0, 0.003 ml mixed with 0.0015 ml precipitation solution, equilibration against 0.5 ml reservoir solution, sitting drop vapour diffusion method, method 1: 0.1 M sodium acetate, pH 4.6, 8% PEG 4000 as precipitant and 0.1 M L-cysteine as additive, triangular-shaped crystals within 10 days, method 2: precipitant solution contains 10% PEG 6000, 2 M NaCl, slow crystal growth, 6 weeks, microseeding into protein solution of 13.5 mg/ml protein, 2 mM Tris-HCl, pH 8.8, 1 day, or hanging drop vapour diffusion method over 1 week, method evaluation, X-ray diffraction and preliminary structure determination and analysis at 2.6 A resolution

Escherichia coli

-

663470

purified enzyme in complex with products pyridoxine 5'-phosphate and phosphate, the enzyme crystals are incubated with 5 mM product solution for 1 h, X-ray diffraction structure determination and analysis at 2.3 A resolution

Escherichia coli

P0A794

666951

purified enzyme, the native enzyme crystals are incubated with 2.5 mM glyceraldehyde 3-phosphate and 2.5 mM 1-deoxy-D-xylulose 5-phosphate for 3 hours, in 10 mM or 100 mM phosphate for 3 days, and for another 3 days in 20 mM 1-deoxy-D-xylulose 5-phosphate, X-ray diffraction structure determination and analysis at 2.3 A resolution

Escherichia coli

P0A794

666014