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2.7.1.1: hexokinase

This is an abbreviated version!
For detailed information about hexokinase, go to the full flat file.

Word Map on EC 2.7.1.1

Reaction

ATP
+
D-glucose
=
ADP
 +
D-glucose 6-phosphate

Synonyms

6-phosphate glucose kinase, AtHXK1, ATP-D-hexose 6-phosphotransferase, ATP-D-hexose-6-phosphotransferase, ATP-dependent hexokinase, ATP: D-glucose 6-phosphotransferase, ATP: D-hexose 6-phosphotransferase, ATP:D-glucose 6-phosphotransferase, ATP:D-hexose 6-phosphotransferase, BmHk, brain form hexokinase, CzHXK1, FgHXK1, FgHXK2, GCK, GK, GKbeta, GlcK, glk, GlkB, glucokinase, glucokinase 1, glucokinase B, glucose ATP phosphotransferase, HaHXK1, hexokinase, hexokinase (phosphorylating), hexokinase 1, hexokinase 2, hexokinase 3, hexokinase 6, hexokinase A, hexokinase D, hexokinase Dor, hexokinase I, hexokinase II, hexokinase III, hexokinase IV, hexokinase PI, hexokinase PII, hexokinase type I, hexokinase type II, hexokinase type IV, hexokinase type IV glucokinase, hexokinase, tumor isozyme, hexokinase-1, hexokinase-10, hexokinase-2, hexokinase-4, hexokinase-5, hexokinase-6, hexokinase-7, hexokinase-8, hexokinase-9, hexokinase-II, hexokinase-like 1, hGK, hGK isoform 1, HK, HK II, HK-1, HK-I, HK-R, HK1, HK2, HK4, HKDC1, HKI, HKII, HKIII, HKL1, HPGLK1, HXK, HXK A, HXK II, Hxk1, HXK10, Hxk2, Hxk3, HXK4, HXK5, HXK6, HXK7, HXK8, HXK9, hxkC, hxkD, kinase, hexo- (phosphorylating), KlHxk1, LGK, LGK2, liver glucokinase, liver glucokinase isoform 2, MODY2 glucokinase, More, muscle form hexokinase, NfGlck, NtHXK1, OsHXK, OsHXK1, OsHXK10, OsHXK2, OsHXK3, OsHXK4, OsHXK5, OsHXK6, OsHXK7, OsHXK9, pHXK, plastid hexokinase, PpHXK1, PpHXK10, PpHXK11, PpHXK2, PpHXK3, PpHXK4, PpHXK5, PpHXK6, PpHXK7, PpHXK8, PpHXK9, Rag5p, ROK hexokinase, ScHK2, ScHXK2, SoHXK1, StHK, STK_23540, StoHK, TbHK1, TbHK2, TcHK, TthHK, type II hexokinase, VIT_18s0001g14230, xprF, Zm.5206, Zm.95484

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.1 hexokinase

Storage Stability

Storage Stability on EC 2.7.1.1 - hexokinase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-180°C, 2 months, stable
-
-20°C, 5 mM sodium-potassium phosphate buffer, pH 7.5, erythrocyte enzyme is more stable than in other buffer systems
-
-20°C, purified enzyme, in the presence of glucose, glycerol and thiol-reducing agents, stable
-
-20°C, purified isoenzymes PI and PII, 25 mM piperazine-HCl, pH 6.5, 50% glycerol, at least several months, minor loss of activity
-
-25°C, over a period of months, stable
-
-80°C, 20 mM Hepes, 1 mM EDTA, 20 mM glucose, 10 mM monothioglycerol, pH 7.5, several months, stable
-
37°C, sodium potassium phosphate buffer, pH 7.5, 9% v/v glycerol, 5 mM glucose, several days, hexokinase Ib is more stable than hexokinase Ia
-
4°C or -20°C, 5 mM sodium potassium phosphate buffer, pH 7.5, 9% v/v glycerol, 5 mM glucose, 3 mM mercaptoethanol, several days, stable
-
4°C, 2.76 M ammonium sulfate, 0.1 M glucose, 10 mM 2-mercaptoethanol, per month, 10% loss of activity
-
4°C, 20 mM Tris-HCl, pH 8, 30% glycerol, 100 mM NaCl, 2 mM CaCl2, 0.1 mM EDTA, 1 mM dithiothreitol, 1 week, about 10% loss of activity
4°C, extensive dialysis of purified enzyme against 0.1 M Tris/HCl, pH 7.6, 1 mM dithiothreitol, 1 mM EDTA, 1 mM sodium azide, 0.2 M ammonium sulfate, stable
-
4°C, polybuffer, over 8 days, stable
-
4°C, several days, progressive loss of activity
-
4°C, sodium potassium phosphate buffer, pH 7.5, 9% v/v glycerol, 5 mM glucose, several days, hexokinase Ia is more stable than hexokinase Ib
-
at least 3 months, stable
-