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2.7.1.100: S-methyl-5-thioribose kinase

This is an abbreviated version!
For detailed information about S-methyl-5-thioribose kinase, go to the full flat file.

Word Map on EC 2.7.1.100

Reaction

ATP
+
S-methyl-5-thio-D-ribose
=
ADP
 +
S-methyl-5-thio-alpha-D-ribose 1-phosphate

Synonyms

5-methylthioribose kinase, ATP:S-methylthioribose kinase, kinase, 5-methylthioribose (phosphorylating), methylthioribose kinase, MTK, MTK1, MtnK, MTR kinase, MTR kinase1, MTRK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.100 S-methyl-5-thioribose kinase

Purification

Purification on EC 2.7.1.100 - S-methyl-5-thioribose kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, aminohexyl-Sepharose, Sephadex g-200, hydroxyapatite Bio-Gel
-
ammonium sulfate, DEAE-Sepharose, 5-(p-aminophenyl)thioribose affinity chromatography
-
ammonium sulfate, partial purified
-
ammonium sulfate, Sephadex G-200, DEAE-cellulose
-
purified from inclusion bodies, gel filtration
recombinant GST-fusion protein from Escherichia coli by glutathione affinity chromatography, the GST-tag is cleaved off
recombinant His-tagged and non-tagged enzymes from Escherichia coli strain BL21(DE3), the latter by anion exchange chromatography, ultracentrifucation and gel filtration
-
recombinant refolded His-tagged enzyme from Escherichia coli by centrifugation, nickel affinity chromatography, ultrafiltration and gel filtration
-