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2.7.1.105: 6-phosphofructo-2-kinase

This is an abbreviated version!
For detailed information about 6-phosphofructo-2-kinase, go to the full flat file.

Word Map on EC 2.7.1.105

Reaction

ATP
+
beta-D-fructose 6-phosphate
=
ADP
+
beta-D-fructose 2,6-bisphosphate

Synonyms

6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase-2, 6-phosphofructo kinase-2/fructose diphosphatase-2 isoenzyme 3, 6-phosphofructo-2-kinase, 6-phosphofructo-2-kinase/2,6-bisphosphatase 3, 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase:glucokinase complex, 6-phosphofructose 2-kinase, 6PF2K/Fru-2,6-P2ase, ATP:D-fructose-6-phosphate 2-phosphotransferase, fructose 6-phosphate 2-kinase, inducible 6-phosphofructo-2-kinase, iPFK2, kinase, 6-phosphofructo-2-(phosphorylating), OsF2KP1, OsF2KP2, Pfk-2, PFK-2/FBPase, PFK-2/FBPase-2, PFK2, PFK2/FBPase2, PFKFB, PFKFB-3, PFKFB1, PFKFB2, PFKFB3, PFKFB4, phosphofructokinase 2, phosphofructokinase-2, phosphofructokinase-2/fructose bisphosphatase-2, T-PFK2, tPFK-2, uPFK-2

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.105 6-phosphofructo-2-kinase

Cloned

Cloned on EC 2.7.1.105 - 6-phosphofructo-2-kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
both parent and double mutant (K472A/K473A) constructs are transformed into the BL21 strain of Escherichia coli, overexpression of PFKFB3 causes no change in glucose metabolism but rather a marked increase in cell proliferation
DNA and amino acid sequence determination and analysis
DNA and amino acid sequence determination and analysis, phylogenetic analysis of the 6-phosphofructo-2-kinase and the fructose-2,6-bisphosphatase domains, expression of His-tagged wild-type and mutant isozymes Tb1, Tb2, and Tb4 in Escherichia coli, poor expression levels and mostly inactive and unstable isozymes, e.g. recombinant Tb2 is inactive
expressed in Escherichia coli
expressed in Escherichia coli, overexpression under the control of the phosphoenolpyruvate carboxykinase promoter in Mus musculus, microinjection of fertilized eggs, C57BL56/SJL receptor females of Mus musculus implanted with microinjected oocytes
expressed in FVB mice, standard embryo microinjection procedures
expression in Escherichia coli
expression in Saccharomyces cerevisiae
expression in Spodoptera frugiperda cells infected with recombinant baculovirus and Escherichia coli
expression of wild-type and mutant enzymes in enzyme-deficient strain DF1020
-
expression of wild-type and mutant enzymes in human HEK-293 cells via adenovirus transfection system, overexpression of the mutant enzyme highly increase the endogenous level of glycolysis and fructose 2,6-bisphosphate in presence of glucagon
-
gene PFKFB1, expression analysis
gene PFKFB1, located on chromosome Xp11.21, the PFKFB1 gene contains 17 exons and encodes 3 different mRNAs L, M and F
gene Pfkfb1, spatiotemporal expression pattern, quantitative real-time PCR expression analysis and phylogenetic analysis
gene PFKFB2
gene PFKFB2, expression analysis
gene PFKFB2, located on chromosome 1q31, encodes the heart isozyme, the PFKFB2 gene encodes four different mRNAs that are derived from different promoters and that vary only in non-coding sequences at the 5'-end
gene Pfkfb2, spatiotemporal expression pattern, quantitative real-time PCR expression analysis and phylogenetic analysis
gene PFKFB3
gene PFKFB3, expression analysis
gene PFKFB3, located on chromosome 10p14-p15, gene PFKFB3 contains at least 19 exons and generates at least 6 different transcripts by alternative splicing. The resulting polypeptides differ in the length of their C-terminal variable regions that are encoded by the last seven exons. Alternative splicing of exon 15 generates the two main isoforms that differ by a short C-terminal sequence: the ubiquitous PFK2 (U-PFK2, 15 exons) and the inducible PFK2 (I-PFK2, 16 exons) isoforms
gene PFKFB3, recombinant expression of GST-tagged isozyme PFKFB3 in Escherichia coli strain BL21(DE3)
gene Pfkfb3, spatiotemporal expression pattern, quantitative real-time PCR expression analysis and phylogenetic analysis
gene PFKFB4, located on chromosome 3p21-p22, the gene contains at least 14 exons, and several splice variants of the PFKFB4 mRNA
gene Pfkfb4, spatiotemporal expression pattern, quantitative real-time PCR expression analysis and phylogenetic analysis
generation of transgenic Arabidopsis lines that overexpress both the fructose 6-phosophate 2-kinase and the fructose 2,6-bisphosphatase domain
-
isoform of enzyme that is induced by proinflammatory stimuli and that is distinguished by the presence of multiple copies of the AUUUA instability motif in its 3' untranslated region is identified and the complete cDNA is cloned and sequenced, expression in eigth human tumor cell lines
mutant expressed in Escherichia coli BL21(DL3)
subcloned into the pET-30b(+) vector, expression in BL21 (DE3) Escherichia coli
subcloning and overexpression of 2 brain enzyme variants HBP1 and HBP2 and of mutant enzymes as His6-tagged proteins in Escherichia coli, expression of enzyme form HBP1 in Spodoptera frugiperda Sf9 insect cells via baculovirus infection system
transfection of RINm5F cell
-
using of recombinant testis enzyme
-
wild-type and mutant protein, expression via adenovirus transfection system in hepatocytes
wild-type enzyme, S466E and S466E/S483E mutants are expressed in Escherichia coli BL21(DE3)pLysE and S483E mutant is expressed in BL21(DE3)pLysS, human embryonic kidney 293 cells are transiently transfected with vectors expressing wild-type heart enzyme
-