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2.7.1.107: diacylglycerol kinase (ATP)

This is an abbreviated version!
For detailed information about diacylglycerol kinase (ATP), go to the full flat file.

Word Map on EC 2.7.1.107

Reaction

ATP
+
1,2-diacyl-sn-glycerol
=
ADP
 +
1,2-diacyl-sn-glycerol 3-phosphate

Synonyms

1,2-diacylglycerol kinase, 1,2-diacylglycerol kinase epsilon, adenosine 5'-triphosphate:1,2-diacylglycerol 3-phosphotransferase, arachidonoyl-specific diacylglycerol kinase, ATP:diacylglycerol phosphotransferase, DAG kinase, DAG-kinase, DAGK, DAGKalpha, DAGKepsilon, DG kinase, DGK, DGK zeta, DGK-1, DGK-3, DGK-3 diacylglycerol kinase, DGK-alpha, DGK-theta, DGK-zeta, DGK1, DGK2, DGK3, DGK4, DGK5, DGK6, DGK7, DGKA, DGKalpha, DgkB, DGKbeta, DGKdelta, DGKE, DGKepsilon, DGKeta, DGKeta1, DGKeta2, DGKeta3, DGKeta4, DGKgamma, DGKiota, DGKkappa, DGKksi, DGKtheta, DGKzeta, diacylglycerol kinase, diacylglycerol kinase (ATP dependent), diacylglycerol kinase 4, diacylglycerol kinase alpha, diacylglycerol kinase beta, diacylglycerol kinase delta, diacylglycerol kinase epsilon, diacylglycerol kinase eta, diacylglycerol kinase theta, diacylglycerol kinase zeta, diacylglycerol kinase-alpha, diacylglycerol kinase-epsilon, diacylglycerol kinase-zeta, diacylglycerol:ATP kinase, diglyceride kinase, epsilonDGK, eye-specific diacylglycerol kinase, kinase (phosphorylating), 1,2-diacylglycerol, kinase, 1,2-diacylglycerol (phosphorylating), OsDAGK1, Rv2252, sn-1,2-diacylglycerol kinase, type V DGK, YegS, YerQ

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.107 diacylglycerol kinase (ATP)

Cloned

Cloned on EC 2.7.1.107 - diacylglycerol kinase (ATP)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a 100fold overproduction is obtained when dgkA is placed on a hybrid plasmid under control of the lambdapl promoter
-
AtDGK2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as NusA/His6-tagged isozyme DGK2 in Escherichia coli strain BL21(DE3)
cDNA subcloned into the EcoRI site of the simian virus 40-based expression vector pSRE
COS-7 cells transfected with DGKksi cDNA express a 117000 Da and a 114000 Da protein. The transfected cells also express increased diacylglycerol kinase activity, which is not altered in the presence of R59949
COS-7 transfection
-
expressed in Arabidopsis thaliana ecotype Col-0
-
expressed in COS-7 cells
expressed in COS-7 cells and in baculovirus-infected Sf21 insect cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli C41(DE3) cells
expressed in Escherichia coli Rosetta2 (DE3) cells
expressed in Escherichia coli strain Rosetta2 (DE3) and baculovirus-infected Sf9 insect cells
expressed in HEK-293, U2OS, MCF-7, Swiss3-T3, MEF, and Phoenix cells
expressed in Sf21 insect cells
expression as His-tagged protein, Escherichia coli
expression in Arabidopsis thaliana protoplasts
-
expression in COS-7 and HEK-293T cells
-
expression in COS-7 cell
expression in COS-7 cell and HeLa cell
-
expression in COS-7 cell, with FLAG-tag
expression in COS-7 cells
expression in Escherichia coli
expression in IIC9 cells
-
expression in lymphocyte
expression in NIH 3T3 cells
-
expression in SF21 cells
-
expression in T lymphocyte and COS-7 cells
expression in tobacco
expression of GFP-tagged or FLAG-tagged wild-type and mutant isozymes theta in COS-7 cells
-
expression of His-tagged wild-type and mutant enzymes
-
expression of the isolated catalytic subunit of isozyme alpha shows 60% of maximal wild-type full length enzyme activity
-
expression of truncated enzyme forms in COS-7 cells
-
expression of wild-type and deletion mutant enzymes in COS-1 cells, expression of wild-type and point mutant enzymes in the enzyme-deficient Saccharomyces cerevisiae strain WY294
-
gene dgk-1alpha, DNA and amino acid sequence determination and analysis of wild-type and mutant enzymes, expression of N-terminally His-tagged wild-type and mutant enzymes and of wild-type enzyme enzyme fused to the maltose binding protein in insect cells via the baculovirus infection system, enzyme is found to 95% in aggregated form
-
gene dgkA, recombinant expression of N-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain WH1061
gene dgkdelta, both splicing variants delta1 and delta2 are recombinantly expressed as FLAG-tagged proteins in enzyme-deficient 3T3-L1 pre-adipocytes, real-time RT-PCR enzyme expression analysis during adipocyte differentiation shows that 3T3-L1 preadipocytes transfected with DGKdelta have no evidence of differentiation to adipocytes
-
GFP-tagged DGKh and DGKg co-expressed in human neuroblastoma cells SH-SY5Y, expression of GST-fusion isozyme DGKalpha and DGKgamma in Escherichia coli strain DH5alpha, expression of GST-fusion isozyme DGKbeta in Spodoptera frugiperda Sf9 cells via baculovirus infection system, expression of GST-fusion isozymes DGKalpha, DGKbeta, and DGKgamma in COS-7 cells
-
high kinase activity is shown in COS cells transfected with either one of the three cDNAs for DGK-I, DGK-II or DGK-III
-
insect cells infected with recombinant baculovirus containing the cDNA
recombinant expression of human His6-tagged DGK isozyme theta from vector pSF-CMV-NH2-His-EKT3 in COS-7 cells
recombinant expression of human p3×FLAG-CMV-DGK isozyme delta2 in COS-7 cells
recombinant expression of human p3×FLAG-CMV-DGK isozyme epsilon in COS-7 cells
recombinant expression of human p3×FLAG-CMV-DGK isozyme eta1 in COS-7 cells
recombinant expression of human p3×FLAG-CMV-DGK isozyme gamma in COS-7 cells
recombinant expression of human p3×FLAG-CMV-DGK isozyme iota1 in COS-7 cells
recombinant expression of human p3×FLAG-CMV-DGK isozyme kappa in COS-7 cells
recombinant expression of human p3×FLAG-CMV-DGK isozyme zeta1 in COS-7 cells
recombinant expression of pig p3×FLAG-CMV-DGK isozyme alpha in COS-7 cells
recombinant expression of rat p3×FLAG-CMV-DGK isozyme beta in COS-7 cells
subcloning into the expression vector pMT-2 and transfection in COS-7 cells results in a 6-7fold increase in diacylglycerol kinase activity
-
the DGKbeta gene can generate several enzyme isoforms which can display different expression levels and subcellular localization but similar enzymatic activities in vitro
transfection of HEK-293 cells, COS-7 cells
-
using a baculovirus-Sf9 insect cell expression system