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2.7.1.107: diacylglycerol kinase (ATP)

This is an abbreviated version!
For detailed information about diacylglycerol kinase (ATP), go to the full flat file.

Word Map on EC 2.7.1.107

Reaction

ATP
+
1,2-diacyl-sn-glycerol
=
ADP
 +
1,2-diacyl-sn-glycerol 3-phosphate

Synonyms

1,2-diacylglycerol kinase, 1,2-diacylglycerol kinase epsilon, adenosine 5'-triphosphate:1,2-diacylglycerol 3-phosphotransferase, arachidonoyl-specific diacylglycerol kinase, ATP:diacylglycerol phosphotransferase, DAG kinase, DAG-kinase, DAGK, DAGKalpha, DAGKepsilon, DG kinase, DGK, DGK zeta, DGK-1, DGK-3, DGK-3 diacylglycerol kinase, DGK-alpha, DGK-theta, DGK-zeta, DGK1, DGK2, DGK3, DGK4, DGK5, DGK6, DGK7, DGKA, DGKalpha, DgkB, DGKbeta, DGKdelta, DGKE, DGKepsilon, DGKeta, DGKeta1, DGKeta2, DGKeta3, DGKeta4, DGKgamma, DGKiota, DGKkappa, DGKksi, DGKtheta, DGKzeta, diacylglycerol kinase, diacylglycerol kinase (ATP dependent), diacylglycerol kinase 4, diacylglycerol kinase alpha, diacylglycerol kinase beta, diacylglycerol kinase delta, diacylglycerol kinase epsilon, diacylglycerol kinase eta, diacylglycerol kinase theta, diacylglycerol kinase zeta, diacylglycerol kinase-alpha, diacylglycerol kinase-epsilon, diacylglycerol kinase-zeta, diacylglycerol:ATP kinase, diglyceride kinase, epsilonDGK, eye-specific diacylglycerol kinase, kinase (phosphorylating), 1,2-diacylglycerol, kinase, 1,2-diacylglycerol (phosphorylating), OsDAGK1, Rv2252, sn-1,2-diacylglycerol kinase, type V DGK, YegS, YerQ

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.107 diacylglycerol kinase (ATP)

Molecular Weight

Molecular Weight on EC 2.7.1.107 - diacylglycerol kinase (ATP)

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
101400
-
x * 101400, DGK-Vtheta, calculation from amino acid sequence
103900
x * 103900, calculation from nucleotide sequence
104000
108000
-
x * 108000, enzyme from Hela cells, SDS-PAGE
109500
x * 109500, calculated
110000
120000
gel filtration
126800
-
x * 126800, DGK-IIeta, calculation from amino acid sequence
128000
x * 128000 DGKeta1, calculation from nucleotide sequence
130000
-
x * 130000, DGK-IIdelta, calculation from amino acid sequence
13245
-
x * 13245, calculation from nucleotide sequence
135000
x * 135000, DGKeta2, calculation from nucleotide sequence
14000
-
x * 14000 Da, SDS-PAGE
140000
x * 79400, about, sequence calculation, x * 140000, about, recombinant NusA/His6-tagged isozyme DGK2, SDS-PAGE
14300
-
x * 14300, SDS-PAGE
15000
-
1 * 15000, SDS-PAGE
152000
-
x * 152000, enzyme form DGK-I, SDS-PAGE
15400
-
gel filtration
250000
-
gel filtration
34000
x * 34000, calculated and SDS-PAGE
37333
x * 37333, calculated, x * 42000, SDS-PAGE
42000
x * 37333, calculated, x * 42000, SDS-PAGE
51000
-
1 * 51000, SDS-PAGE
58000
-
x * 58000, enzyme form DGK-III, SDS-PAGE
64000
isoform DGKepsilon
68000
-
less than, sucrose density gradient centrifugation
75000
76000
-
gel filtration
78000
-
1 * 78000, SDS-PAGE
79400
x * 79400, about, sequence calculation, x * 140000, about, recombinant NusA/His6-tagged isozyme DGK2, SDS-PAGE
80000
-
SDS-PAGE
82200
-
x * 82200, DGK-Ialpha, calculation from amino acid sequence
82600
-
x * 82600, DGK-Ialpha, calculation from amino acid sequence
82700
-
x * 82700, DGK-Ialpha, calculation from amino acid sequence
86000
-
gel filtration
88500
-
x * 88500, DGK-Igamma, calculation from amino acid sequence
89000
-
x * 89000, DGK-Igamma, calculation from amino acid sequence
90000
isoform DGKbeta, SDS-PAGE
90300
-
x * 90300, DGK-Ibeta, calculation from amino acid sequence
95000
-
gel filtration
additional information
-
the molecular weight of the peak fraction of enzyme from supernatant is 125000 Da and the average molecular weight is 160000 Da, suggesting that more than one species might be present