2.7.1.11: 6-phosphofructokinase
This is an abbreviated version!
For detailed information about 6-phosphofructokinase, go to the full flat file.
Word Map on EC 2.7.1.11
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2.7.1.11
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citrate
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glycogen
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amp
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glucose-6-phosphate
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2,6-bisphosphate
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aldolase
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phosphorylase
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phosphoenolpyruvate
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erythrocyte
-
pentose
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glucokinase
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gluconeogenesis
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malate
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creatine
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hexose
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glyceraldehyde-3-phosphate
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phosphoglycerate
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fructose-2,6-bisphosphate
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enolase
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fructose-1,6-bisphosphatase
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gluconeogenic
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vastus
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carboxykinase
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6-phosphogluconate
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lateralis
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6-phosphofructo-2-kinase
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phosphoglucose
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triose
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phosphoglucomutase
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pfkfb3
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soleus
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1,6-bisphosphatase
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mgatp
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phosphocreatine
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fbpase
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m-type
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fructose-1,6-diphosphate
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myoglobinuria
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bisphosphatase
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glycogenosis
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2.7.1.1
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1,6-diphosphate
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liver-type
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entner-doudoroff
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2,3-diphosphoglycerate
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3-hydroxyacyl-coa
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mcardle
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synthesis
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heterotropic
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biotechnology
-
analysis
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glycogenolytic
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myophosphorylase
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medicine
- 2.7.1.11
- citrate
- glycogen
- amp
- glucose-6-phosphate
- 2,6-bisphosphate
- aldolase
- phosphorylase
- phosphoenolpyruvate
- erythrocyte
- pentose
- glucokinase
-
gluconeogenesis
- malate
- creatine
- hexose
- glyceraldehyde-3-phosphate
- phosphoglycerate
- fructose-2,6-bisphosphate
- enolase
- fructose-1,6-bisphosphatase
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gluconeogenic
-
vastus
-
carboxykinase
- 6-phosphogluconate
- lateralis
- 6-phosphofructo-2-kinase
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phosphoglucose
- triose
- phosphoglucomutase
- pfkfb3
- soleus
-
1,6-bisphosphatase
- mgatp
- phosphocreatine
- fbpase
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m-type
- fructose-1,6-diphosphate
- myoglobinuria
-
bisphosphatase
- glycogenosis
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2.7.1.1
- 1,6-diphosphate
-
liver-type
-
entner-doudoroff
- 2,3-diphosphoglycerate
- 3-hydroxyacyl-coa
- mcardle
- synthesis
-
heterotropic
- biotechnology
- analysis
-
glycogenolytic
- myophosphorylase
- medicine
Reaction
Synonyms
1-phosphofructokinase, 2-keto-3-deoxygluconate kinase, 6-phosphofructo 1-kinase, 6-phosphofructo-1-kinase, 6-phosphofructokinase I, 6-phosphofructokinase, platelet type, 6-phosphofructose 1-kinase, 6-phosphofructose-1-kinase, A/GTP-PFK, ADP-dependent phosphofructokinase, APE0012, At2g22480, At4g26270, At4g29220, At4g32840, At5g56630, At5g61580, ATP-dependent 6-phosphofructokinase, ATP-dependent 6-phosphofructokinase isozyme 2, ATP-dependent PFK, ATP-dependent phosphofructokinase, ATP-PFK, ATP: D-fructose-6-phosphate-1-phosphotransferase, ATP:D-fructose-6-phosphate-1-phosphotransferase, D-fructose-6-phosphate 1-phosphotransferase, fructose 6-phosphate kinase, fructose 6-phosphokinase, hosphofructokinase-1, HVO_A0328, KDG kinase, KDGal kinase, KDGK-1, KDGK-2, kinase, phosphofructo- (phosphorylating), LmPFK, muscle-type phosphofructokinase, nucleotide triphosphate-dependent phosphofructokinase, OC_Os04g39420, PFK, PFK-1, Pfk-2, PFK-L, PFK-M, PFK-P, PFK1, PFK2, PFK3, PFK4, PFK5, PFK6, PFK7, PFKA, PFKA1, PFKA2, PFKA3, pfkB, PFKL, PFKM, PFKMS, PFKP, PfpA4, phospho-1,6-fructokinase, phosphofructo-1-kinase, phosphofructokinase, phosphofructokinase 1, phosphofructokinase M, phosphofructokinase-1, phosphofructokinase-2, phosphofructokinase-M, phosphofructokinase-P, phosphohexokinase, TbPFK, TcPFK
ECTree
2.7.1.11 6-phosphofructokinaseAdvanced search results
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results (Inhibitors
Inhibitors on EC 2.7.1.11 - 6-phosphofructokinase
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INHIBITOR 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
1,3-diphosphoglycerate
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0.385 mM and 0.005 mM, 50% inhibition of PFK I and PFK II respectively, phosphate relieves from inhibition
2',3'-dialdehyde ATP
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irreversibly modifies a single lysine residue in the ATP-inhibitory site, thereby locking PFK in a permanantly inhibited state without affecting the integrity of the active site
Antibodies against rabbit muscle enzyme
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not rabbit erythrocyte, leukocyte or platelet enzyme
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ascorbate
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inhibition by ascorbate is PFK-1 concentration dependent. Ascorbate does not inhibit above 200 nM PFK-1. It is concluded that ascorbate inhibits PFK-1 dimers (and perhaps monomers) but not PFK-1 tetramers
clotrimazole
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clotrimazole alone induces dimerization of the enzyme reducing the population of tetramers, which is not observed when calmodulin is also present. Since PFK dimers are less active than tetramers, this can explain the inhibitory effect of clotrimazole
D-fructose 6-phosphate
inhibition occurs at low concentrations of fructose 6-phosphate
Dithionitrobenzoic acid
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0.06 mM, 80% inhibition of PFK III, reversed by 2-mercaptoethanol, dithiothreitol or reduced glutathione
Li2CO3
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about 75% residual activity at 20 mM, about 40% residual activity at 40 mM, about 10% residual activity at 100 mM, less than 3% residual activity at 200 mM
Li2SO4
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about 55% residual activity at 100 mM, about 30% residual activity at 200 mM
lithium acetate
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about 80% residual activity in the presence of 0.009 mM lithium acetate
MgADP-
addition of MgADP has little effect the specific activity of the enzyme, but diminishes the homotropic cooperativity of fructose 6-phosphate binding, reducing the Hill numbers from 1.6 to 1
p-Chloromercurisulfonic acid
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0.025 mM, 80% inhibition of PFK III, reversed by 2-mercaptoethanol, dithiothreitol or reduced glutathione
Protein factor
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19000 Da protein promotes Zn2+ or Fe2+-dependent dissociation into inactive protomers, maximal inactivation at 0.001-0.02 mM Zn2+, inactivation is abolished at higher Zn2+ concentrations, Ca2+, Mg2+, Mn2+ can substitute for Zn2+ or Fe2+ only at millimolar concentrations, potency in descending order: Mn2+, Mg2+, Ca2+, inactivation can be reversed by the addition of ATP, fructose 1,6-bisphosphate, or fructose 2,6-bisphosphate
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2-phosphoglycerate
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2 mM and 3.2 mM, 50% inhibition of cytosolic PFK at pH 8.0 and PH 7.2, respectively, 0.5 mM and 0.4 mM, 50% inhibition of plastid PFK at pH 8.0 and pH 7.2, respectively
2-phosphoglycerate
Dunaliella marina
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1 mM, complete inhibition, phosphate relieves, kinetics
2-phosphoglycerate
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0.032 mM and 0.004 mM, 50% inhibition of PFK I and PFK II respectively, phosphate relieves from inhibition
2-phosphoglycerate
-
1.3 mM, 50% inhibition of cytosolic PFK, no inhibition in the presence of 5 or 25 mM phosphate
3-phosphoglycerate
Dunaliella marina
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1 mM, complete inhibition, phosphate relieves, 0.5 mM, 50% inhibition, sigmoidal inhibition curve; inhibition kinetics
ADP
-
most potent inhibitor of cytosolic isozyme, 0.4 mM and 4 mM, 50% inhibition of cytosolic and plastid PFK at pH 8.0
ADP
substrate inhibition above 2 mM; substrate inhibition above 2 mM; substrate inhibition above 2 mM
ADP
-
1.9 mM and 0.0006 mM, 50% inhibition of PFK I and PFK II respectively, phosphate relieves from inhibition
AMP
1 mM and 4 mM, 20% and 40% inhibition in the presence of 5 mM fructose 6-phosphate and Mg2+
AMP
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at high fructose 6-phosphate concentrations, activates at low fructose 6-phosphate concentrations
ATP
-
above 0.05 mM, inhibition is more pronounced at pH 6.6 but occurs also at pH 8.0, inhibition is reversed by equivalent amounts of AMP
ATP
in contrast to the native PFK1 enzyme, the 49 kDa PFK1 fragment is highly sensitive to ATP inhibition (5fold lower activity at the 1 mM ATP than at the 0.1 mM concentration)
ATP
-
cytosolic isoenzyme is more sensitive to inhibition at pH 8.0 than pH 7.2, Mg2+ alleviates inhibition
ATP
-
2 mM, 50% inhibition of cytosolic PFK at pH 8.0, 5 mM, 50% inhibition of plastid PFK at pH 8.0
ATP
inhibition of fructose 1-phosphate phosphorylation above 0.08 mM ATP
ATP
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1 mM, almost complete inhibition of native PFK, phosphorylated PFK is less sensitive to inhibition
ATP
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above 1 mM at 0.33 mM fructose 6-phosphate and 2 mM Mg2+, inhibition at a Mg/ATP ratio below 2; ADP partially reverses inhibition; fructose 6-phosphate partially reverses inhibition; Mg2+ partially reverses inhibition
ATP
-
fructose 2,6-bisphosphate partially reverses inhibition; phosphate and AMP partially reverse inhibition
ATP
-
ADP partially reverses inhibition; cAMP partially reverses inhibition; fructose 1,6-bisphosphate partially reverses inhibition; phosphate and AMP partially reverse inhibition
ATP
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ADP partially reverses inhibition; fructose 6-phosphate partially reverses inhibition; phosphate and AMP partially reverse inhibition
ATP
-
0.02 mM and 0.94 mM, 50% inhibition at 0.0001 mg/ml and at 0.0006 mg/ml PFK respectively, 3.23 mM, 50% inhibition at 0.0006 mg/ml PFK in the presence of 10% polyethylene glycol
ATP
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muscle PFK, strong inhibition at pH 7.1, weak inhibition at pH 7.6-8.5, not inhibited at pH 9.0
ATP
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the inhibition of enzyme activity by ATP (above 1 mM) is abolished in the presence of calmodulin
ATP
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cAMP partially reverses inhibition; fructose 6-phosphate partially reverses inhibition; pH-dependent inhibition; phosphate and AMP partially reverse inhibition
ATP
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7.5 mM, 98% inhibition; fructose 2,6-bisphosphate partially reverses inhibition
ATP
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fructose 1,6-bisphosphate partially reverses inhibition; phosphate and AMP partially reverse inhibition
ATP
-
inhibition of plastid PFK above 5 mM at pH 8.0 and above 0.5 mM at pH 7.2
ATP
-
at physiological concentration; at physiological pH, the enzyme is allosterically inhibited by near-physiological concentrations of its co-substrate ATP, which induces cooperativity, i.e. reduces the affinity for the substrate fructose 6-phosphate. Inhibition by ATP is reinforced by citrate and H+
ATP
-
allosteric inhibition by high ATP concentrations. The inhibitory effects of high ATP concentration can be reversed by D-fructose 2,6-bisphosphate and AMP
ATP
-
0.004 mM and 0.003 mM, 50% inhibition at 20°C and 6°C, respectively, normoxic turtles
aurintricarboxylic acid
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0.0002 mM, 50% inhibition at pH 7.3, reversed by addition of allosteric activators, i.e., fructose 2,6-bisphosphate or AMP, no inhibition at pH 8.0
Ca2+
-
depending upon total Mg2+-concentration, kinetics, cytosolic isozyme
citrate
-
strong inhibition; synergistic with ATP; synergistic with NH4+; synergistic with phosphate and AMP
citrate
the native 85000 Da enzyme is moderately inhibited by citrate, the 49000 Da shorter fragment of PFK1 proves to be completely resistant to inhibition by citrate
citrate
when 5 mM of citrate is added a moderate reduction for about 1020% is recorded in the homogenate of TE22 and TE23 strain, while in A158 strain much stronger reduction of PFK1 specific activity, for approximately 40% is observed
citrate
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activates the enzyme in the absence of phosphate and inhibits the enzyme in the presence of phosphate
citrate
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with glucose 1,6-bisphosphate or fructose 1,6-bisphosphate as activator
citrate
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almost complete inhibition at 1 mM citrate in the absence of D-fructose 2,6-bisphosphate
citrate
-
mild inhibitory effects, but only at concentrations exceeding 2 mM
citrate
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0.23 and 0.17 mM mM, 50% inhibition at 20° and 6°C, respectively, normoxic turtles, 0.3 mM, 50% inhibition at 20°C and 6°C, anoxic turtles
D-fructose 1,6-bisphosphate
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0.02 mM, 50% inhibition in the presence of physiological concentrations of ATP, AMP, phosphate and fructose 6-phosphate
D-fructose 1,6-bisphosphate
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0.02 mM, 50% inhibition at 100 mM fructose 6-phosphate in the absence of fructose 2,6-bisphosphate, inhibition is reversed by fructose 2,6-bisphosphate
D-Glucose 1,6-bisphosphate
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0.08 mM, 50% inhibition in the presence of physiological concentrations of ATP, AMP, phosphate and fructose 6-phosphate, inhibition is relieved by 50% by 0.008 mM fructose 2,6-bisphosphate
diphosphate
1 mM and 4 mM, 20% and 50% inhibition in the presence of 5 mM fructose 6-phosphate and 5 mM Mg2+
diphosphate
-
0.1 mM, 80% inhibition, nucleotide diphosphates, i.e. ADP, GDP or TDP, partially alleviate inhibition
GDP
-
activates the enzyme in the absence of phosphate and inhibits the enzyme in the presence of phosphate
isocitrate
-
mild inhibitory effects, but only at concentrations exceeding 2 mM
K+
-
about 30% residual activity of 30 nM PFK-1 in the presence of 0.2 M K+
Lactate dehydrogenase
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lactate dehydrogenase suspended in 3.2 M ammonium sulfate inhibits 30 nM PFK-1 resulting in a more than 50% inhibition of activity
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Lactate dehydrogenase
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lactate dehydrogenase suspended in 3.2 M ammonium sulfate inhibits 30 nM PFK-1 resulting in a more than 50% inhibition of activity
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malate
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mild inhibitory effects, but only at concentrations exceeding 2 mM
Mg2+
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free form, citrate reverses, independent of MgATP-concentration, kinetics, cytosolic isozyme
Na+
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about 70% residual activity of 30 nM PFK-1 in the presence of 0.2 M Na+
NH4+
-
muscle PFK, weak, at high concentrations, activation at very low concentrations
NH4+
-
about 40% residual activity of 30 nM PFK-1 in the presence of 0.2 M NH4+
palmitoyl-CoA
-
low micromolar inhibitor, MgAMP and MgADP but not MgATP protect the enzyme against inhibition by palmitoyl-CoA. Acyl-protein thioesterase-1 reverses palmitoyl-CoA-mediated enzyme inhibition
phosphate
-
inhibition at 10-100 mM, concentrations at 1- 5 mM relieve the influence of other inhibitors such as phosphoenolpyruvate
phosphate
Dunaliella marina
-
above 5 mM; kinetics; the enzyme is both activated and inhibited by phosphate, depending on fructose 6-phosphate/phosphate-ratio
phosphate
-
25 mM, 50% and 57% inhibition of PFK-1 at pH 6.6 and pH 7.2, respectively
phosphate
slight inhibition; slight inhibition; slight inhibition
phosphoenolpyruvate
-
0.1 mM, 50% inhibition at low pH; phosphate relieves
phosphoenolpyruvate
-
effective inhibition at 0.005-0.02 mM, fructose 6-phosphate relieves inhibition; most potent inhibitor
phosphoenolpyruvate
-
0.04 mM and 0.05 mM, 50% inhibition of plastid PFK at pH 8.0 and pH 7.2, respectively; most potent inhibitor
phosphoenolpyruvate
Dunaliella marina
-
1 mM, complete inhibition, 0.08 mM, 50% inhibition; inhibition kinetic; phosphate relieves
phosphoenolpyruvate
I153V mutation has a substantial positive impact on the magnitude of inhibition by phosphoenolpyruvate
phosphoenolpyruvate
-
0.0023 mM and 0.0009 mM, 50% inhibition of PFK I and PFK II respectively, phosphate relieves from inhibition
phosphoenolpyruvate
-
0.22 mM, 50% inhibition of cytosolic PFK in the presence of 1 mM fructose 6-phosphate, phosphate relives inhibition
phosphoenolpyruvate
-
0.1 mM and 10 mM, allosteric inhibition, almost completely reversed by 2.5 mM ADP
phosphoenolpyruvate
addition leads to a decease in fructose 6-phosphate binding affinity and in heterotropically induced homotropic cooperativity in fructose 6-phosphate binding, the Hill numbers increase from 1.6 to above 2.5 without decrease in specific activity
Phosphoglycolate
-
0.006 mM and 0.003 mM, 50% inhibition of PFK I and PFK II respectively, phosphate relieves from inhibition
SO42-
-
20 mM, 50% inhibition of cytosolic and 77% inhibition of plastid PFK at pH 7.2, not at pH 8.0
additional information
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not inhibited by ATP; not inhibited by fructose 1,6-bisphosphate
-
additional information
the active 49 kDa PFK1 fragment is not inhibited by citrate
-
additional information
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the active 49 kDa PFK1 fragment is not inhibited by citrate
-
additional information
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not inhibited by fructose 1,6-bisphosphate
-
additional information
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not inhibited by fructose 1,6-bisphosphate
-
additional information
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not inhibited by ADP; not inhibited by Ca2+; not inhibited by fructose 1,6-bisphosphate
-
additional information
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cytosolic PFK is not inhibited by GTP; not inhibited by fructose 2,6-bisphosphate
-
additional information
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not inhibited by ATP; not inhibited by fructose 1,6-bisphosphate
-
additional information
Dunaliella marina
-
not inhibited by ADPglucose, dithiothreitol, gluconate 6-phosphate, glucose 1-phosphate; not inhibited by NH4+ and K+
-
additional information
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not inhibited by fructose 1,6-bisphosphate; not inhibited by ITP, fumarate, tricarballylic acid, CoA, acetyl-CoA
-
additional information
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not inhibited by fructose 1,6-bisphosphate; not inhibited by pyruvate; not inhibited by ribulose 5-phosphate
-
additional information
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not inhibited by fructose 1,6-bisphosphate; not inhibited by ITP, fumarate, tricarballylic acid, CoA, acetyl-CoA
-
additional information
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product inhibition of reverse reaction
-
additional information
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not inhibited by fructose 1,6-bisphosphate
-
additional information
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high insulin concentration decreases enzyme activity in intact cells
-
additional information
-
not inhibited by ATP; not inhibited by fructose 1,6-bisphosphate
-
additional information
-
not inhibited by ATP; not inhibited by fructose 1,6-bisphosphate
-
additional information
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not inhibited by 2-phosphoglycerate; not inhibited by 3-phosphoglycerate; not inhibited by ADP; not inhibited by fructose 1,6-bisphosphate
-
additional information
-
not inhibited by 2-phosphoglycerate; not inhibited by 3-phosphoglycerate; not inhibited by ADP; not inhibited by fructose 1,6-bisphosphate
-
additional information
-
not inhibited by 2-phosphoglycerate; not inhibited by fructose 2,6-bisphosphate; not inhibited by glutamine, glutamate
-
additional information
-
not inhibited by fructose 1,6-bisphosphate; not inhibited by ITP, fumarate, tricarballylic acid, CoA, acetyl-CoA; photooxidation yields a new heart enzyme species that is no longer sensitive to ATP
-
additional information
-
not inhibited by AMP; not inhibited by cAMP; not inhibited by phosphate
-
additional information
-
not inhibited by AMP; not inhibited by cAMP; not inhibited by phosphate; not inhibited by pyruvate
-
additional information
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not inhibited by fructose 1,6-bisphosphate; not inhibited by ITP, fumarate, tricarballylic acid, CoA, acetyl-CoA; photooxidation yields a new heart enzyme species that is no longer sensitive to ATP
-
additional information
-
acetyl-CoA, malonyl-CoA, palmitoylcarnitine, and palmitic acid in the presence of CoASH are without effect on enzyme activity
-
additional information
-
heart enzyme, not inhibited by cis-aconitate, L-isocitrate, alpha-ketoglutarate, succinate, fumarate, malate, tricarnallylic acid, CoASH, or acetyl-CoASH; not inhibited by fructose 1,6-bisphosphate; not inhibited by ITP, fumarate, tricarballylic acid, CoA, acetyl-CoA; photooxidation yields a new heart enzyme species that is no longer sensitive to ATP
-
additional information
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not inhibited by fructose 1,6-bisphosphate; not inhibited by GTP; not inhibited by ITP, fumarate, tricarballylic acid, CoA, acetyl-CoA
-
additional information
-
not inhibited by 6-phosphogluconate and phosphocreatine
-
additional information
-
not inhibited by 3-phosphoglycerate; not inhibited by fructose 2,6-bisphosphate; not inhibited by Na+
-
additional information
-
not inhibited by cAMP; not inhibited by UDP, UDPglucose
-
additional information
-
not inhibited by 2-phosphoglycerate; not inhibited by 3-phosphoglycerate; not inhibited by ADP; not inhibited by fructose 1,6-bisphosphate
-
additional information
-
effect of low temperature or anoxia on inhibition kinetics
-
additional information
-
not inhibited by fructose 1,6-bisphosphate; not inhibited by fructose 2,6-bisphosphate; not inhibited by phosphate
-
