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2.7.1.127: inositol-trisphosphate 3-kinase

This is an abbreviated version!
For detailed information about inositol-trisphosphate 3-kinase, go to the full flat file.

Word Map on EC 2.7.1.127

Reaction

ATP
+
1D-myo-inositol 1,4,5-trisphosphate
=
ADP
 +
1D-myo-inositol 1,3,4,5-tetrakisphosphate

Synonyms

1D-myo-inositol-trisphosphate 3-kinase, Arg82, ArgRIII, D-myo-inositol 1,4,5-trisphosphate 3-kinase, GsIP3K-A, HsIP3K-A, inositol (1,4,5) trisphosphate 3 kinase B, inositol (1,4,5) trisphosphate 3-kinase, inositol 1,4,5-trisphosphate 3-kinase, inositol 1,4,5-trisphosphate 3-kinase A, inositol 1,4,5-trisphosphate 3-kinase B, inositol 1,4,5-trisphosphate 3-kinase C, inositol 1,4,5-trisphosphate 3-kinases, inositol 1,4,5-trisphosphate kinase, inositol 1,4,5-trisphosphate kinase 2, inositol 1,4,5-trisphosphate-3-kinase-A, inositol polyphosphate multikinase, inositol trisphosphate 3-kinase B, inositol(1,4,5)P3 3-kinase, inositol(1,4,5)trisphosphate 3-kinase, inositol(1,4,5)trisphosphate 3-kinases, inositol-1,4,5-trisphosphate 3-kinase, inositol-1,4,5-trisphosphate 3-kinase A, inositol-1,4,5-trisphosphate 3-kinase-A, inositol-1,4,5-trisphosphate-3-kinase, inositol-1,4,5-trisphosphate-3-kinase A, inositol-1,4,5-trisphosphate-3-kinase-A, inositol-trisphosphate 3-kinase B, Ins(1,4,5)P(3) 3-kinase B, Ins(1,4,5)P3 3-kinase, Ins(1,4,5)P3 3-kinase isoform B, Ins(1,4,5)P3 kinase, InsP3 3-kinase, InsP3 3-kinase A, InsP3 3-kinase B, InsP3 3-kinase C, InsP3K, InsP3KB, InsP3Kinase, IP3 3-kinase, IP3 3-kinase 2, IP3-3K, IP3K, IP3K-A, IP3K-B, IP3K-C, IP3K1, IP3K2, IP3KA, IP3KB, IP3KC, IP3kin, Ipk2, IPMK, ITPKA, Itpkb, Itpkc, kinase (phosphorylating), inositol 1,4,5-trisphosphate 3-, More, RnIP3K-C, wavy

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.127 inositol-trisphosphate 3-kinase

Purification

Purification on EC 2.7.1.127 - inositol-trisphosphate 3-kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
114fold
-
195fold
-
27800fold
-
4662fold
-
943fold
-
By phosphocellulose.
calmodulin-Sepharose affinity chromatography
-
cerebellum, partial
-
elution of recombinant Itpka isozyme with 0.5% SDS and 0.1 mM EGTA from calmodulin affinity column
His6-tagged fragment comprising residues 151-461 from Escherichia coli by nickel affinity chromatography, unlabeled or selenomethionine-labeled recombinant residues 185-459 of the catalytic domain of isozyme A fused to GST from Escherichia coli by glutathione affinity chromatography, removal of the GST-tag by recombinant TEV protease, further purification by a heparin affinity chromatography, and gel filtration
-
isoenzyme B
native enzyme partially by nuclear fraction preparation
partial
purification of GST-inositol 1,4,5-trisphosphate-3-kinase-A protein
Purification of IP3K isoform A and point mutant are performed by phosphocellulose and calmodulin affinity chromatography.
-
Purification of IP3K isoform A is performed by phosphocellulose and calmodulin affinity chromatography.
Purification of IP3K isoform B is performed by phosphocellulose and calmodulin affinity chromatography.
purified in the presence of SDS as a doublet of a MW of approximately 50000
-
recombinant GST-fusion isoform B comprising residues 108-170 by glutathione affinity chromatography, recombinant truncated Strep-tagged isozyme B by affinity chromatography
recombinant IP3K overexpressed in Escherichia coli
-
recombinant isoenzyme A overexpressed in CHO cells
-
recombinant isoforms A and B, overexpressed in B31 rat fibroblast, isoform A: 11005fold, isoform B: 43806fold
-
recombinant isozyme ITPKA from Escherichia coli strain BL21 by phosphocellulose chromatography and calmodulin affinity chromatography
recombinant N-terminally GST-tagged isozyme ITPKA from Escherichia coli strain BL21 C41lys by glutathione affinity chromatography
recombinant N-terminally His-tagged and S-tagged C-terminal catalytic domain 3KA-cat30, comprising residues Ser185-Arg459, from Escherichia coli strain BL21(DE3) by nickel and S-protein affinity chromatography, and gel filtration
-
recombinant wild-type or mutant fragments comprising the catalytic domain and the CaM binding domain of isozyme IP3K-A and IP3K-B from Escherichia coli strain BL21(DE3) by phosphocellulose and CaM affinity chromatography
-
recombinant wild-type or mutant fragments comprising the catalytic domain and the CaM binding domain of isozyme IP3K-A from Escherichia coli strain BL21(DE3) by phosphocellulose and CaM affinity chromatography
-
unlabeled or selenomethionine-labeled recombinant residues 185-459 of the catalytic domain of isozyme A fused to GST from Escherichia coli by glutathione affinity chromatography, removal of the GST-tag by recombinant TEV protease, further purification by a second step of glutathione affinity chromatography, adsorption chromatography, and gel filtration
-