2.7.1.136: macrolide 2'-kinase

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For detailed information about macrolide 2'-kinase, go to the full flat file.

Reaction

Synonyms

macrolide 2'-phosphotransferase, macrolide 2'-phosphotransferase II, macrolide 2'-phosphotransferase type I, macrolide 2'-phosphotransferase type II, Mph, MPH (2'), MPH(2'), MPH(2')-I, MPH(2')-II, mphA, mphB, phosphotransferase, macrolide 2'-

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.1 Phosphotransferases with an alcohol group as acceptor

                2.7.1.136 macrolide 2'-kinase

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Results	in table
1	Activating Compound
7	AA Sequence
1	Application
1	CAS Registry Number
8	Cloned(Commentary)
2	Cofactor
2	Crystallization (Commentary)
4	General Information
11	Inhibitors
1	kcat/KM [mM/s]
1	Ki Value [mM]
1	KM Value [mM]
2	Localization
10	Metals/Ions
1	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
34	Organism
1	pH Optimum
6	Protein Variants
1	Purification (Commentary)
1	Reaction
1	Reaction Type
24	Reference
12	Specific Activity [micromol/min/mg]
1	Storage Stability
97	Substrates and Products (Substrate)
1	Subunits
20	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Turnover Number [1/s]

Specific Activity

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Specific Activity on EC 2.7.1.136 - macrolide 2'-kinase

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SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

0.0007

Escherichia coli

-

purified recombinant mutant H205A, substrate oleandomycin

661781

0.04

Escherichia coli

-

purified recombinant wild-type enzyme, substrate roxithromycin or rokitamycin

661781

0.05

Escherichia coli

-

purified recombinant wild-type enzyme, substrate josamycin or tylosin

661781

0.052

Escherichia coli

-

purified recombinant mutant H198A, substrate oleandomycin

661781

0.056

Escherichia coli

-

purified recombinant mutant H205N, substrate oleandomycin

661781

0.06

Escherichia coli

-

purified recombinant wild-type enzyme, substrate troleandomycin, clarithromycin, azithromycin, or erythromycin

661781

0.1

Escherichia coli

-

purified recombinant wild-type enzyme, substrate oleandomycin

661781

0.16

Escherichia coli

-

purified recombinant wild-type enzyme, substrate kitasamycin

661781

0.21

Escherichia coli

-

purified recombinant wild-type enzyme, substrate spiramycin

661781

additional information

3 entries

additional information

Escherichia coli

-

substrate specificities of the mutant enzymes

661781

additional information

Escherichia coli

Q47396

genetic map of the resistance plasmid pRSB105, functional antibiotic resistance regions of plasmid pRSB105 characterized, plasmid pRSB105 harbors the macrolide resistance gene mph, encoding a macrolide 2'-phosphotransferase, transcript analysis by RT-PCR, erythromycin resistance analyzed by determining values for minimum inhibitory concentration, mph gene shown to contribute to erythromycin resistance

690516

additional information

uncultured bacterium

Q5W3B4

accessory region on plasmid pRSB111 contains a new macrolide resistance operon, mph(E) gene encodes a macrolide phosphotransferase required for high-level macrolide resistance, resistance analyzed by determining values for minimum inhibitory concentration

690460