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2.7.1.137: phosphatidylinositol 3-kinase

This is an abbreviated version!
For detailed information about phosphatidylinositol 3-kinase, go to the full flat file.

Word Map on EC 2.7.1.137

Reaction

ATP
+
1-phosphatidyl-1D-myo-inositol
=
ADP
 +
1-phosphatidyl-1D-myo-inositol 3-phosphate

Synonyms

class 3 phosphatidylinositol 3-kinase vacuolar protein sorting 34, class I phosphoinositide 3-kinase, class III phosphatidylinositol 3-kinase, class III phosphoinositide 3-kinase, class III PI3K, HsC2-PI3K, hVps34, hVps34 PI 3-kinase, kinase (phosphorylating), phosphatidylinositol 3-, kinase, phosphatidylinositol 3- (phosphorylating), lipid kinase Vps34, mammalian vacuolar protein sorting 34 homologue, More, p110a, p110delta I PI3K., p110g-related PI 3-kinase, p85/p110 phosphoinositide 3-kinase, p85/p110 type phosphatidylinositol kinase, p85a phosphoinositide 3-kinase, phosphatidyl-inositol-3-kinase, phosphatidylinositide 3-kinase, phosphatidylinositide 3-kinase C2alpha, phosphatidylinositol 3 kinase, phosphatidylinositol 3'-kinase, phosphatidylinositol 3-kinase, phosphoinositide 3'-kinase, phosphoinositide 3-kinase, phosphoinositide 3-kinase Dp110, phosphoinositide 3-kinase gamma, phosphoinositide-3-kinase, PI 3-K, PI 3-kinase, PI-3-K, PI-3K, PI3 K, PI3 kinase, PI3-K, PI3-K-related protein MEC1, PI3-kinase, PI3K, PI3K type III, PI3Kalpha, PI3Kbeta, PI3Kgamma, PI3K_59F, PI3K_68D, PIK3, PIK3C3, protein sorting mutant 34 protein, PtdIns 3'-kinase, PtdIns 3-kinase, PtdIns 3-kinase vacuolar protein sorting 34, receptor-linked phosphatidylinositol 3-kinase, Rickettsia intracellular secreted kinase-1, Risk1, RT0135, TcVps34, type III phosphatidylinositol 3-kinase, vacuolar protein sorting 34, VPS34, Vps34p

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.137 phosphatidylinositol 3-kinase

Cloned

Cloned on EC 2.7.1.137 - phosphatidylinositol 3-kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
C2 domain-containing phosphoinositide 3-kinase, HsC2-PI3K
CaVPS34 gene under the control of its own promoter is not able to complement the temperature-sensitive growth of Saccharomyces cerevisiae vps34, overexpression of CaVPS34 is sufficient to rescue the temperature-sensitive vps34 phenotype, suggesting a functional conservation in Candida albicans
class Ia isozymes, DNA sequence determination and analysis, expression of His-tagged isozymes or P85 and p110 subunits alone in Spodoptera frugiperda Sf9 insect cells utilizing the baculovirus infection system
-
class II phosphoinositide 3-kinase with a C2 domain, cloned from a U937 monocyte cDNA library, expression in mammalian and insect cells
class II phosphoinositide 3-kinase, PI 3-kinase C2b, with a C2 domain is cloned from a U937 monocyte cDNA library and expressed in mammalian and insect cells
cloning of a human phosphoinositide 3-kinase with a C2 domain that displays reduced sensitivity to the inhibitor wortmannin
-
cloning, structural organization, and chromosomal localization of the mouse PI3-kinase p110a gene
-
construction of a tetracycline-inducible expression system by introducing a regulatory plasmid, which constitutively expresses tetracycline-sensitive transactivator, and a response plasmid, containing a tetracycline response element in form of a heptameric repeat of the tetR binding site, under control of a minimal CMV promotor, into Jurkat cells, individual and coexpression of the Myc-tagged enzyme subunits in the Jurkat cell system, determination of expression levels, overview
-
DNA and amino acid sequence determination and analysis, recombinant expression of the His-tagged TcVps34 in Escherichia coli strain BL21(DE3) and complementation of an enzyme-deficient Saccharomyces cerevisiae knockout mutant strain
expressed in Escherichia coli
-
expression of GST-tagged PI3K SH3 domain in Escherichia coli strain Bl21
-
expression of the 110000 Da subunit in Sf9 insect cells and in COS-1 cells
expression of the catalytic subunit p110alpha of the wild-type and mutant enzymes in COS-1 cells
-
expression of the complete coding sequence as a glutathione S-transferase fusion protein in Sf9 cells
expression of the GRP1 domain, comprising residues 263-380, in Escherichia coli
-
expression of the p85 subunit and a related p85beta protein by using baculovirus expression system
-
expression of the wild-type p110 alpha protein in CHO cells
expression of wild-type and mutant HA-tagged PI3K isozyme gamma and of subunit p110 in COS-7 cells, stimulation of the protein kinases Erk, JNK, and PKB
-
expression of wild-type VPS34 and PI3-K-related protein MEC1 in a enzyme-knockout mutant strain, functional complementation analysis, overview
-
gene AtVPS34, genetic structure determination, sequence determination and genotyping, overview
gene PIK3R2
gene RT1035, recombinant expression of wild-type and mutant enzymes in HeLa cells
genotyping
-
p110 expressed in insect cells possesses Pl3-kinase activity and associates with p85 alpha into an active p85 alpha-p110 complex that binds the activated colony-stimulating factor 1 receptor. p110 expressed in COS-1 cells is catalytically active only when complexed with p85 alpha
phosphoinositide-3 kinase isotype, p110 gamma
PI 3-kinase p170
-
PI3K-C2 gamma gene locus is mapped to the distal region of mouse chromosome 6 in a region of homology with human chromosome 12p
PIK3C2G is mapped to chromosome 12
stable expression of GFP-tagged Atg14 in NIH3T3 cells, transient co-expression of N-terminally Myc-, HA-, or FLAG-tagged Atg14, Beclin 1, and UVRAG in HEK-293T cells
transient co-expression of N-terminally Myc-, HA-, or FLAG-tagged Beclin 1 in HEK-293T cells
transient co-expression of N-terminally Myc-, HA-, or FLAG-tagged UVRAG in HEK-293T cells