2.7.1.145: deoxynucleoside kinase

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For detailed information about deoxynucleoside kinase, go to the full flat file.

Reaction

Synonyms

AGCK, AgdNK, BmdNK, D. melanogaster deoxynucleoside kinase, deoxyribonucleoside kinase, deoxyribonucleoside kinase , deoxyribonucleoside phosphotransferase, Dm-dNK, DmdNK, dNK, kinase (phosphorylating), deoxynucleoside, More, ms-dNK, multi-substrate deoxyribonucleoside kinase, multifunctional deoxynucleoside kinase, multispecific deoxynucleoside kinase, multisubstrate deoxyribonucleoside kinase, nucleoside triphosphate: deoxyribonucleoside 5'-phosphotransferase, pyrimidine deoxyribonucleoside kinase, white stripe leaf8, WSL8, Xen-PyK

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.1 Phosphotransferases with an alcohol group as acceptor

                2.7.1.145 deoxynucleoside kinase

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Results	in table
28	AA Sequence
21	Application
1	CAS Registry Number
28	Cloned(Commentary)
23	Cofactor
7	Crystallization (Commentary)
1	Expression
11	General Information
1	General Stability
18	IC50 Value
47	Inhibitors
9	kcat/KM [mM/s]
22	Ki Value [mM]
140	KM Value [mM]
8	Localization
15	Metals/Ions
19	Molecular Weight [Da]
49	Natural Substrates/ Products (Substrates)
49	Organism
4	Pathway
68	PDB ID
5	pH Optimum
54	Protein Variants
14	Purification (Commentary)
7	Reaction
1	Reaction Type
47	Reference
10	Source Tissue
14	Specific Activity [micromol/min/mg]
282	Substrates and Products (Substrate)
18	Subunits
75	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
100	Turnover Number [1/s]

Purification

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Purification on EC 2.7.1.145 - deoxynucleoside kinase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Drosophila melanogaster

-

636839, 636840, 673848, 675450

chelating Sepharose column chromatography and Superdex S200 gel filtration

Drosophila melanogaster

Q9XZT6

762219

native enzyme from cell lines in a 5-step procedure, recombinant GST-fusion enzyme from Escherichia coli strain KY895 by GST-affinity chromatography

Anopheles gambiae

Q86LB8

662967

recombinant C-terminally His8-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography

Bombyx mori

Q9BKL3

662607

recombinant C-terminally His8-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography, the His-tag is cleaved off by thrombin

Drosophila melanogaster

-

662607

recombinant C-terminally His8-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 by nickel affinity chromatography

Drosophila melanogaster

-

661583

recombinant enzyme

Drosophila melanogaster

Q9XZT6

636842

recombinant full length and N-terminally truncated His-tagged enzymes, the His-tags are cleaved off

Xenopus laevis

Q8UVZ9

662607

recombinant His10-tagged enzyme from Spodoptera frugiperda SF9 cells by nickel affinity chromatography and gel filtration

Drosophila melanogaster

Q9XZT6

738739

recombinant wild-type and mutant enzymes as glutathione-S-transferase fusion proteins from Escherichia coli by glutathione affinity chromatography

Drosophila melanogaster

-

662990

recombinant wild-type and mutant GST-fusion enzymes from Escherichia coli by glutathione affinity chromatography

Drosophila melanogaster

-

661629