2.7.1.146: ADP-specific phosphofructokinase

This is an abbreviated version!
For detailed information about ADP-specific phosphofructokinase, go to the full flat file.

Word Map on EC 2.7.1.146

2.7.1.146

hyperthermophilic

embden-meyerhof

archaeon

methanococcus

jannaschii

amp

furiosus

thermococcus

atp-pfks

phosphofructokinases

ppi-dependent

fructose-1,6-bisphosphate

sulfate-reducing

archaeoglobus

fulgidus

pyrophosphate-dependent

zilligii

thermococcales

eucarya

horikoshii

adp-forming

Reaction

Synonyms

ADP dependent phosphofructokinase, ADP-6-phosphofructokinase, ADP-dependent 6-phosphofructokinase, ADP-dependent glucokinase, ADP-dependent glucokinase/phosphofructokinase, ADP-dependent PFK, ADP-dependent phosphofructokinase, ADP-GK, ADP-Pfk, ancGK/PFK, AncMsPFK/GK, bifunctional ADP-dependent phosphofructokinase/glucokinase, MevePFK/GK, MJ1604, MjPFK/GK, MmazPFK/GK, MmPFK/GK, More, PFK, PFK-ADP, pfkC, PhPFK, TK0376, TLPFK

ECTree

2 Transferases

2.7 Transferring phosphorus-containing groups

2.7.1 Phosphotransferases with an alcohol group as acceptor

2.7.1.146 ADP-specific phosphofructokinase

Advanced search results

Do not include text mining results

Include

results (more...)

Include

results (more...)

Results	in table
6	Activating Compound
254	AA Sequence
1	CAS Registry Number
22	Cloned(Commentary)
18	Cofactor
8	Crystallization (Commentary)
54	General Information
2	General Stability
5	IC50 Value
39	Inhibitors
71	kcat/KM [mM/s]
10	Ki Value [mM]
110	KM Value [mM]
1	Localization
83	Metals/Ions
21	Molecular Weight [Da]
53	Natural Substrates/ Products (Substrates)
78	Organism
10	Pathway
7	PDB ID
19	pH Optimum
6	pH Range
1	pI Value
35	Protein Variants
18	Purification (Commentary)
1	Reaction
2	Reaction Type
55	Reference
2	Source Tissue
22	Specific Activity [micromol/min/mg]
1	Storage Stability
130	Substrates and Products (Substrate)
16	Subunits
104	Synonyms
1	Systematic Name
14	Temperature Optimum [°C]
3	Temperature Range [°C]
8	Temperature Stability [°C]
70	Turnover Number [1/s]

Purification

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Purification on EC 2.7.1.146 - ADP-specific phosphofructokinase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide

Go to Purification (commentary) Search

6 entries

1818fold to homogeneity by ultracentrifugation, anion exchange, ADP affinity, and cation exchange chromatography, and gel filtration

Archaeoglobus fulgidus

661677

by using nickel-nitrilotriacetic acid affinity chromatography and gel filtration

Pyrococcus horikoshii

O59355

698924

Ni-NTA column chromatography

Pyrococcus horikoshii

partial

recombinant enzyme 3.5fold from Escherichia coli strain BL21-CodonPlus(DE3)-RIL by heat treatment at 80°C for 30 min, anion exchange chromatography, and dialysis, followed by hydrophobic interaction chromatography, and again dialysis

Thermococcus kodakarensis

Q5JD05

761560

recombinant enzyme from Escherichia coli strain JM109 by ammonium sulfate fractionation, adsorption and ion exchange chromatography, and gel filtration

Thermococcus litoralis

Q977Q3

660573

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and anion exchange chromatography

3 entries

recombinant N-terminally His6-tagged enzyme from Escherichia coli strains BL21(DE3) and BL21-C41(DE3) by heat treatment at 50°C for 30 min, nickel affinity chromatography, dialysis, and anion exchange chromatography

Methanohalobium evestigatum

D7E8P3

760433

recombinant N-terminally His6-tagged enzyme from Escherichia coli strains BL21(DE3) and BL21-C41(DE3) by nickel affinity chromatography, and dialysis

Methanococcoides burtonii

Q12WB9

760433