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2.7.1.148: 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase

This is an abbreviated version!
For detailed information about 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, go to the full flat file.

Word Map on EC 2.7.1.148

Reaction

ATP
+
4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
=
ADP
 +
2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol

Synonyms

4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase, 4-(cytidine-5-diphospho)-2-C-methyl-D-erythritol kinase, 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, 4-diphosphocytidyl-2-C-methylerythritol 2-kinase, 4-diphosphocytidyl-2C-methyl-D-erythritol 2-kinase, 4-diphosphocytidyl-2C-methyl-D-erythritol kinase, AaIspE, BsIspE, CcCMK1, CDP-ME, CDP-ME kinase, CDP-methylerythritol kinase, CDPME kinase, CDPMEK, CMK, EcIspE, GbCMK1, GbCMK2, IspDF, IspE, More, PvIspE, Ripening-associated protein pTOM41, TwCMK, YchB

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.148 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase

Purification

Purification on EC 2.7.1.148 - 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Hi-trap chelating columns are used for the purification of recombinant 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase
-
recombinant enzyme
recombinant GST-tagged enzyme from Escherichia coli strain DH5alpha by glutathione affinity chromatography, tag cleavage through Prescission protease, followed by anion exchange chromatography, and gel filtration
recombinant His-tagged enzyme from strain BL21(DE3) to homogeneity by nickel affinity and anion exchange chromatography
-
recombinant His6-tagged enzyme by nickel affinity chromatography
-
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage through thrombin, followed by anion exchange chromatography, and gel filtration
recombinant selenomethionine-labeled enzyme from Escherichia coli strain B834(DE3) by ammonium sulfate fractionation, hydrophobic interaction and heparin affinity chromatography, gel filtration, and ultrafiltration
-
The gene is preceded by a His6 tag to enable purification of the recombinant protein via metal-chelating affinity chromatography. The polyhistidine tag is removed by thrombin-mediated proteolysis, followed by purification with anion-exchange chromatography. Purity of the sample is assessed by SDS-PAGE and MALDI-TOF MS.