2.7.1.156: adenosylcobinamide kinase

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For detailed information about adenosylcobinamide kinase, go to the full flat file.

Reaction

Synonyms

adenosylcobinamide kinase, adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase, AdoCbi kinase/AdoCbi-phosphate guanylyltransferase, ATP/GTP:AdoCbi kinase, bifunctional CobU enzyme, CobP, CobU, CobU AdoCbi kinase, CobU protein, GTP:adenosylcobinamide-phosphate guanylyltransferase, GTP:AdoCbi-P kinase, GTP:AdoCbi-phosphate guanylyltransferase, NTP:adenosylcobinamide kinase, NTP:AdoCbi kinase

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.1 Phosphotransferases with an alcohol group as acceptor

                2.7.1.156 adenosylcobinamide kinase

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Results	in table
1	Activating Compound
21074	AA Sequence
1	CAS Registry Number
5	Cloned(Commentary)
4	Cofactor
3	Crystallization (Commentary)
1	General Stability
5	Inhibitors
2	KM Value [mM]
6	Molecular Weight [Da]
31	Natural Substrates/ Products (Substrates)
15	Organism
1	Oxidation Stability
6	Pathway
3	PDB ID
2	pH Optimum
1	pH Range
3	pI Value
4	Protein Variants
6	Purification (Commentary)
3	Reaction
2	Reaction Type
15	Reference
2	Source Tissue
5	Specific Activity [micromol/min/mg]
1	Storage Stability
56	Substrates and Products (Substrate)
4	Subunits
36	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Range [°C]
2	Turnover Number [1/s]

Purification

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Purification on EC 2.7.1.156 - adenosylcobinamide kinase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

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Salmonella enterica subsp. enterica serovar Typhimurium

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644435, 644436, 644437, 644438

CobU protein is overproduced and purified. Cells from a 4-liter culture of the overproducing strain are lysed using a French press. After the addition of ammonium sulfate, fast protein liquid chromatography is used to isolate CobU protein from the clarified cell extract in two additional steps, hydrophobic interaction chromatography and dye ligand chromatography. Protein concentration was determined by the Bradford method.

Salmonella enterica subsp. enterica serovar Typhimurium

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674340

recombinant enzyme from Escherichia coli by ammonium sulfate fractionation, hydrophobic interaction chromatography on a phenyl resin, and ultrafiltration

Salmonella enterica subsp. enterica serovar Typhimurium

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663316