2.7.1.158: inositol-pentakisphosphate 2-kinase

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For detailed information about inositol-pentakisphosphate 2-kinase, go to the full flat file.

Word Map on EC 2.7.1.158

2.7.1.158

hexakisphosphate

phytate

multikinase

1,2,3,4,5,6-hexakisphosphate

tetrakisphosphate

5/6-kinase

diagnostics

Reaction

1D-myo-inositol 1,3,4,5,6-pentakisphosphate

Synonyms

ASP1, At5g42810, AtIP5 2-K, AtIPK1, CnIpk1, Glyma04g03240, Glyma06g03310, GmIpk1, GmlPk1, Gsl1p, inositol 1,3,4,5,6-pentakis phosphate 2-kinase, inositol 1,3,4,5,6-pentakisphosphate 2-kinase, inositol hexakisphosphate kinase 1, inositol kinase, inositol pentakisphosphate 2-kinase, inositol polyphosphate kinase, inositol polyphosphate kinase-1, inositol polyphosphate kinase/phosphotransferase, inositol-1,3,4,5,6-pentakisphosphate 2-kinase, ins(1,3,4,5,6)P5 2-kinase, Ins5 2-kinase, InsP5 2-kinase, InsP6-kinase, IP5 2-K, IP5 2-kinase, IP5K, IP6K, IP6K1, Ipk1, Ipk1p, IPP2K, mIP5 2-K, Plc1p, rIPK1, scIpk1, spIpk1-C, StITPK1, StITPKalpha, ZmIPK1, ZmIPK1A, ZmIPK1B

ECTree

2 Transferases

2.7 Transferring phosphorus-containing groups

2.7.1 Phosphotransferases with an alcohol group as acceptor

2.7.1.158 inositol-pentakisphosphate 2-kinase

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Results	in table
3	Activating Compound
3687	AA Sequence
1	Application
1	CAS Registry Number
22	Cloned(Commentary)
11	Cofactor
4	Crystallization (Commentary)
31	Disease/ Diagnostics
32	General Information
2	General Stability
1	IC50 Value
11	Inhibitors
4	kcat/KM [mM/s]
33	KM Value [mM]
20	Localization
14	Metals/Ions
5	Molecular Weight [Da]
36	Natural Substrates/ Products (Substrates)
43	Organism
8	Pathway
37	PDB ID
8	pH Optimum
1	pH Range
2	pI Value
27	Protein Variants
15	Purification (Commentary)
1	Reaction
3	Reaction Type
39	Reference
70	Source Tissue
2	Specific Activity [micromol/min/mg]
1	Storage Stability
65	Substrates and Products (Substrate)
16	Subunits
108	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
1	Temperature Range [°C]
16	Turnover Number [1/s]

Purification

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Purification on EC 2.7.1.158 - inositol-pentakisphosphate 2-kinase

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2 entries

a glutathione affinity column is used to capture intact GST-ZmIPK1. The purified GST-ZmIPK1 fusion is cleaved with thrombin and further purified by a sequential affinity and ion-exchange chromatography. ZmIPK1 protein is eluted at 0.1 M NaCl on a high resolution Mono Q column with an estimated purity of 95%. Identity of the purified protein is confirmed via trypsin digestion followed by matrix-assisted laser deposition/ionization (MALDI) time-of-flight mass spectrometry analysis and comparison of the MALDI fingerprint to the amino acid sequences deduced from ZmIPK1.

Zea mays

A4GWX8, A6YH13

676634

by batch-elution from Ni-NTA affinity resin, Analysis by SDS-PAGE gel and Western Blot using an anti-His antibody. Purity of protein is >98% estimated by SDS-PAGE.

Solanum tuberosum

A4H2J0

686761

expression in Escherichia coli

Zea mays

A4GWX8, A6YH13

676634

glutathione agarose bead chromatography

Mus musculus

Q6PD10

737654

Ni-NTA bead chromatography

recombinant

recombinant enzyme

recombinant His-tagged enzyme from Escherichia coli strain BL21 (Lys) DE3 by nickel affinity chromatography

Homo sapiens

Q9H8X2

760326

recombinant LSLt-tagged enzyme from Escherichia coli strain BL21 Star (DE3) by heparin affinity chromatography and gel filtration followed by tag cleavage with TEV protease and again heparin affinity chromatography and gel filtration. The recombinant GST-tagged enzyme from Sf21 insect cells is purified by glutathione affinity chromatography and tag cleavage with PreScission protease, followed by heparin affinity chromatography and gel filtration

Mus musculus

Q6P1C1

762323

recombinant N-terminally His5-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and tag cleavage through TEV protease, followed by another step of nickel affinity chromatography, and gel filtration

Cryptococcus neoformans var. grubii

J9VKS8

761763