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2.7.1.159: inositol-1,3,4-trisphosphate 5/6-kinase

This is an abbreviated version!
For detailed information about inositol-1,3,4-trisphosphate 5/6-kinase, go to the full flat file.

Word Map on EC 2.7.1.159

Reaction

ATP
+
1D-myo-inositol 1,3,4-trisphosphate
=
ADP
 +
1D-myo-inositol 1,3,4,5-tetrakisphosphate

Synonyms

1,3,4-trisphosphate 5/6-kinase, 5/6 kinase, 5/6-kinase, At2G43980, AtITPK1, AtITPK2, AtITPK3, AtITPK4, GmItpk2, inositol 1,3,4 tris phosphate 5/6 kinase-2, inositol 1,3,4 trisphosphate kinase-2, inositol 1,3,4-triphosphate 5/6 kinase, inositol 1,3,4-triphosphate 5/6-kinase, inositol 1,3,4-trisphosphate 5/6 kinase-2, inositol 1,3,4-trisphosphate 5/6-kinase, inositol 1,3,4-trisphosphate 5/6-kinase 1, inositol 1,3,4-trisphosphate 5/6-kinase 4, inositol 1,3,4-trisphosphate 5/6-kinase6, inositol 1,4,5 trisphosphate 3/6 kinase, inositol-1,3,4-trisphosphate kinase, Ins(1,3,4)P3 5/6-kinase, IP56K, Ipk2, ITPK, Itpk-1, ITPK1, ITPK2, ITPK3, More, Os09g0518700, OsITL1, OsITPK6

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.159 inositol-1,3,4-trisphosphate 5/6-kinase

Cloned

Cloned on EC 2.7.1.159 - inositol-1,3,4-trisphosphate 5/6-kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Cloning and transformation of tobacco plants (Nicotiana tabacum L. cv. K326)
A1KXK8
DNA and amino acid sequence determination and analysis, expression as GST-tagged enzyme in bacteria
DNA and amino acid sequence determination and analysis, expression as tagged enzyme in Escherichia coli
-
DNA and N-terminal amino acid sequence determination and analysis
-
DNA and N-terminal amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli
expression in 293 cells, expression in Spodoptera frugiperda Sf21 cells using the baculovirus infection system
-
expression in Escherichia coli
expression in HeLa cells and in HEK-293 cells
-
expression in Sf9 cells and HEK-293 cells
-
expression of GST-tagged wild-type enzyme and of mutant enzymes in Escherichia coli
expression of His-tagged enzyme in Escherichia coli strain BL21
expression of the enzyme tagged with 6 repeats of Myc at the N-terminus in Spodoptera frugiperda Sf21 cells using the baculovirus infection system, expression of His6-tagged and of FLAG-tagged enzyme in Escherichia coli strain BL21(DE3), co-overexpression of the enzyme with CSN1 in HeLa cells
-
gene itpk, quantitative RT-PCR enzyme expression analysis
-
gene ITPK2, expression analysis of wild-type and mutant enzymes by quantitative real-time PCR analysis
gene Itpk2, genetic organization and gene structure, isozyme sequence comparisons, in-silico analysis of the promoter region of ITPKs, the promoter region of GmITPK2 shows the presence of two MYB binding elements for drought inducibility and one for abscisic acid response, quantitative RT-PCR enzyme expression analysis. The His5-tagged GmITPK2-expressing recombinant Escherichia coli strain BL21(DE3) cells show tolerance to drought and salinity and thus demonstrate the functional redundancy of the gene across taxa
gene itpk3-1, genotyping, 108 different accessions, the Arabidopsis accessions differ in their response to Zn deficiency, quantitative real-time PCR enzyme expression anaysis, overview
gene ositpk6, sequence comparisons, expression analysis
in Escherichia coli Rosetta, transformed with pET28a and pET28c containing 3 kinase cDNAs. Vector-encoded hexa-histidine (His) tags are added to all proteins.
single copy Itpk2 gene, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged GmITPK2 in Escherichia coli strain BL21(DE3)
stable overexpression in HEK-293 cells
-