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2.7.1.160: 2'-phosphotransferase

This is an abbreviated version!
For detailed information about 2'-phosphotransferase, go to the full flat file.

Word Map on EC 2.7.1.160

Reaction

2'-phospho-[ligated tRNA]
+
NAD+
=
mature tRNA
 +
ADP-ribose 1'',2''-phosphate
 +
nicotinamide

Synonyms

2'-phosphotransferase, ApeTpt1, AtPt, CTHT_0025470, HeLa cell 2'-phosphotransferase, human TRPT1, KptA, kptA1, mTPT1, NAD+-dependent 2'-phosphotransferase, NAD-dependent RNA 2'-phosphotransferase, RNA 2'-phosphotransferase, RslTpt1, Tpt1, Tpt1p, tRNA 2'-phosphotransferase, Trpt1, yeast 2'-phosphotransferase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.160 2'-phosphotransferase

Engineering

Engineering on EC 2.7.1.160 - 2'-phosphotransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H22A
-
lethal in vivo
R125A
-
lethal in vivo
R21A
-
lethal in vivo
R69A
-
lethal in vivo
R16A/H17A/R64A/R119A
-
site-directed mutagenesis, the R16A, H17A, R64A, and R119A mutations are deemed lethal in vivo
R64A
-
inactive step 2-defective RslTpt1-R64A mutant
R16A/H17A/R64A/R119A
-
site-directed mutagenesis, the R16A, H17A, R64A, and R119A mutations are deemed lethal in vivo
-
C85R
-
no accumulation of the reaction intermediate
D34A
-
no accumulation of the reaction intermediate
F72A
-
temperature sensitive
H117A
H117A-T119A
-
lethal in vivo
H140A
-
no accumulation of the reaction intermediate
H140A/H142A
-
no accumulation of the reaction intermediate
H140A/V141A/H142A
-
no accumulation of the reaction intermediate
H142A
H24A
-
lethal in vivo
H24N
-
lethal in vivo
H24Q
-
lethal in vivo
I167L/D168K
-
no accumulation of the reaction intermediate
K16A
-
colony size indistinguishable from that of wild-type TPT1 strain
K69A
-
colony size indistinguishable from that of wild-type TPT1 strain
K69A/R71S
-
this mutant produces a substantial amount of the reaction intermediate
M136G
-
no accumulation of the reaction intermediate
N185A
-
no accumulation of the reaction intermediate
Q88A/H90A/S91A
-
no accumulation of the reaction intermediate
Q88Y
-
no accumulation of the reaction intermediate
R138A
R138K
-
colony size indistinguishable from that of wild-type TPT1 strain
R138Q
-
lethal in vivo
R158A
-
colony size indistinguishable from that of wild-type TPT1 strain
R158H
-
no accumulation of the reaction intermediate
R23A
-
lethal in vivo
R23A/H24A
-
no accumulation of the reaction intermediate
R23K
-
lethal in vivo
R23Q
-
lethal in vivo
R71A
-
lethal in vivo
R71K
-
lethal in vivo
R71Q
-
grows slower than wild-type, temperature sensitive
S155A
-
no accumulation of the reaction intermediate
S15A/K16A
-
no accumulation of the reaction intermediate
S183A
-
no accumulation of the reaction intermediate
T119A
-
colony size indistinguishable from that of wild-type TPT1 strain
Y38A
-
colony size indistinguishable from that of wild-type TPT1 strain
additional information
generation and analysis of diverse mutant strains derived from Saccharomyces cerevisiae strain W303. The essential functions of TRL1 and TPT1 in budding yeast are bypassed by expressing prespliced, intronless versions of the 10 normally intron-containing tRNAs, indicating this repair pathway does not have additional essential functions. Expression of intronless tRNAs fails to rescue the growth of cells with deletions in components of the SEN complex, implying an additional essential role for the splicing endonuclease. The trl1DELTA and tpt1DELTA mutants accumulate tRNA and HAC1 splicing intermediates indicative of RNA repair defects and are hypersensitive to drugs that inhibit translation. tpt1DELTA mutants grow in the presence of tunicamycin despite reduced accumulation of spliced HAC1 mRNA, while failure to induce the unfolded protein response occurs in trl1DELTA cells grown with tunicamycin is lethal owing to their inability to ligate HAC1 after its cleavage by Ire1. Tpt1DELTA phenotype, overview