2.7.1.161: CTP-dependent riboflavin kinase

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For detailed information about CTP-dependent riboflavin kinase, go to the full flat file.

Reaction

Synonyms

CTP-dependent riboflavin kinase, Methanocaldococcus jannaschii Mj0056, MJ0056, myRbkR, pyRbkR, RbkR, RibK, riboflavin kinase, taRbkR

ECTree

2 Transferases

2.7 Transferring phosphorus-containing groups

2.7.1 Phosphotransferases with an alcohol group as acceptor

2.7.1.161 CTP-dependent riboflavin kinase

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Results	in table
2747	AA Sequence
1	CAS Registry Number
7	Cloned(Commentary)
4	Cofactor
4	Crystallization (Commentary)
41	General Information
2	General Stability
8	Inhibitors
8	KM Value [mM]
11	Metals/Ions
4	Molecular Weight [Da]
14	Natural Substrates/ Products (Substrates)
18	Organism
5	Pathway
8	PDB ID
2	pH Optimum
1	pH Stability
5	Purification (Commentary)
1	Reaction
17	Reference
1	Source Tissue
33	Substrates and Products (Substrate)
8	Subunits
58	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Stability [°C]

Purification

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Purification on EC 2.7.1.161 - CTP-dependent riboflavin kinase

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recombinant

Methanocaldococcus jannaschii

Q60365

680522

recombinant C-terminally His6-tagged enzyme from Escherichia coli strain Rosetta2(DE3)pLysS by nickel affinity chromatography, gel filtration, hydrophobic interaction chhromatography, and ultrafiltration

Thermoplasma acidophilum

Q9HJA6

762041

recombinant enzyme

Methanocaldococcus jannaschii

Q60365

680522

recombinant N-terminally His6-Smt3-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, the His6-Smt3-tag is then cleaved from the purified RbkR protein by digestion with Ulp1 protease

2 entries