Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.7.1.163: hygromycin B 4-O-kinase

This is an abbreviated version!
For detailed information about hygromycin B 4-O-kinase, go to the full flat file.

Word Map on EC 2.7.1.163

Reaction

ATP
+
hygromycin B
=
ADP
 +
4-O-phosphohygromycin B

Synonyms

Hm phosphotransferase, HPH, Hph5, HPT, hptII, hygromycin B phosphotransferase, hygromycin B4-O-phosphohygromycin, hygromycin phosphotransferase, hygromycin-B kinase, hygromycin-phosphotransferase, HyR

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.163 hygromycin B 4-O-kinase

Engineering

Engineering on EC 2.7.1.163 - hygromycin B 4-O-kinase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A118V
D20G/A118V/S225P/Q226L/T246A
-
site-directed mutagenesis, mutant gene hpt5, mutant enzyme is stable at up to 67°C in contrary to the wild-type enzyme
Q226L
S225P
S52T
-
site-directed mutagenesis, the mutation does not confer thermostability at 55°C to the mutant enzyme
T246A
W238C
-
site-directed mutagenesis, the mutation does not confer thermostability at 55°C to the mutant enzyme
additional information