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2.7.1.17: xylulokinase

This is an abbreviated version!
For detailed information about xylulokinase, go to the full flat file.

Word Map on EC 2.7.1.17

Reaction

ATP
+
D-xylulose
=
ADP
 +
D-xylulose 5-phosphate

Synonyms

1-deoxy-D-xylulokinase, ATP/polyphosphate xylulokinase, D-xululokinase, D-xylulokinase, D-xylulose kinase, DXK, Huta_2446, kinase (phosphorylating), xylulo, kinase, xylulo- (phosphorylating), TM0116, XK, XK-1, XKS1, XyiH, XYL3, XylB, xylokinase(phosphorylating), xylulokinase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.17 xylulokinase

Purification

Purification on EC 2.7.1.17 - xylulokinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
apo-enzyme and in complex with D-xylulose, ATP plus D-xylulose or 5-deoxy-5-fluoro-D-xylulose, nickel affinity column chromatography and Superdex 200 gel filtration
harvested cells resuspended in 50 mM Tris/HCl pH 7.8, solution after ultrasonically disruption centrifuged at 10000 *g for 30 min at 4°C, supernatant passed through immobilized metal affinity chromatography (5 ml) using the His-tag introduced into the C-terminal region of XK, purified enzyme dialyzed against 50 mM Tris/Hcl (pH 7.8) containing 12.5% (w/v) glycerol
-
Ni-NTA column chromatography and Sephacryl S200 gel filtration
nickel affinity column chromatography and Superdex 200 gel filtration
-
partial
partial purification using Sepharose-Q column chromatography
-
recombinant enzyme
-