2.7.1.170: anhydro-N-acetylmuramic acid kinase

This is an abbreviated version!
For detailed information about anhydro-N-acetylmuramic acid kinase, go to the full flat file.

Reaction

Synonyms

1,6-anhydro-N-acetylmuramic acid kinase, anhMurNAc kinase, anhydro-N-acetylmuramic acid kinase, AnmK, YdhH

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.1 Phosphotransferases with an alcohol group as acceptor

                2.7.1.170 anhydro-N-acetylmuramic acid kinase

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Results	in table
17628	AA Sequence
6	Cloned(Commentary)
6	Cofactor
4	Crystallization (Commentary)
13	General Information
5	Inhibitors
3	Ki Value [mM]
6	KM Value [mM]
2	Localization
7	Metals/Ions
2	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
37	Organism
4	Pathway
8	PDB ID
2	pH Optimum
2	Protein Variants
3	Purification (Commentary)
6	Reaction
10	Reference
19	Substrates and Products (Substrate)
3	Subunits
23	Synonyms
1	Systematic Name
1	Turnover Number [1/s]

Crystallization

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Crystallization on EC 2.7.1.170 - anhydro-N-acetylmuramic acid kinase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

crystal structure analysis

Escherichia coli

P77570

738435

analysis of several crystal structures

Pseudomonas aeruginosa

Q9I5Q5

738435

AnmK in complex with 1,6-anhydro-N-acetylmuramic acid and ADP, hanging drop vapor diffusion method, using 25% (w/v) polyethylene glycol 4000, 0.2 M (NH4)2SO4, and 0.1 M tri-sodium citrate (pH 5.6)

Pseudomonas aeruginosa

Q9I5Q5

715588

purified recombinant enzyme bound to a nonhydrolyzable ATP analogue (AMPPCP) and 1,6-anhydroMurNAc, hanging drop vapor diffusion method, for enzyme AnmK bound to AMPPCP, 6.5 mg/ml protein in 600 mM NaCl, 2 mM AMPPCP, 4 mM MgCl2, 0.5 mM TCEP, and 20 mM Tris, pH 8.0, is mixed with an equal volume of mother liquor containing 26% PEG 4000, 0.2 M MgCl2, 0.1 M Tris, pH 9.0, for enzyme AnmK bound to AMPPCP and the anhMurNAc sugar, AnmK s first co-crystallized with AMPPCP by mixing equal volumes of mother liquor containing 24% PEG 4000, 0.2 M MgCl2, 0.1 M Tris, pH 8.2, with the enzyme at 6.5 mg/ml in 600 mM NaCl, 2 mM AMPPCP, 4 mM MgCl2, 0.5 mM TCEP, 20 mM Tris, pH 8.0, a single crystal of the AnmK-AMPPCP complex is then transferred to a drop of reservoir buffer supplemented with 8 mM anhMurNAc, 2 mM AMPPCP, and 4 mM MgCl2 and soaked for 1 h, at room temperature, X-ray diffraction structure determination and analysis at 1.67-1.84 A resolution

Pseudomonas aeruginosa

Q9I5Q5

738636