2.7.1.174: diacylglycerol kinase (CTP)

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For detailed information about diacylglycerol kinase (CTP), go to the full flat file.

Reaction

Synonyms

CTP-dependent DAG kinase, CTP-dependent diacylglycerol kinase, DAG kinase, DGK1, Dgk1p, diacylglycerol kinase (CTP dependent), More

ECTree

2 Transferases

2.7 Transferring phosphorus-containing groups

2.7.1 Phosphotransferases with an alcohol group as acceptor

2.7.1.174 diacylglycerol kinase (CTP)

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Results	in table
8	Activating Compound
8	AA Sequence
1	CAS Registry Number
3	Cloned(Commentary)
3	Cofactor
1	Expression
16	General Information
1	IC50 Value
17	Inhibitors
1	Ki Value [mM]
1	KM Value [mM]
9	Localization
5	Metals/Ions
9	Natural Substrates/ Products (Substrates)
9	Organism
5	Pathway
3	pH Optimum
1	pH Range
2	Posttranslational Modification
20	Protein Variants
1	Reaction
7	Reference
2	Specific Activity [micromol/min/mg]
1	Storage Stability
17	Substrates and Products (Substrate)
4	Subunits
21	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
2	Temperature Stability [°C]

Cloned

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Cloned on EC 2.7.1.174 - diacylglycerol kinase (CTP)

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expressed in Escherichia coli BL21(DE3) cells

Saccharomyces cerevisiae

Q12382

761456

gene DGK1

Saccharomyces cerevisiae

Q12382

693092

gene DGK1, the DGK1 promoter is substituted with the inducible GAL1/10 promoter in the low copy YCplac111 and high copy YEplac181 vectors, expression of wild-type and mutant enzymes, induction of wild-type DGK1 gene expression from high copy number plasmid YEplac181-GAL1/10-DGK1 results in a massive increase in DAG kinase activity. Temperature sensitivity of dgk1DELTA cells overexpressing DGK1 and its mutant alleles

Saccharomyces cerevisiae

Q12382

687738