2.7.1.178: 2-dehydro-3-deoxyglucono/galactono-kinase
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For detailed information about 2-dehydro-3-deoxyglucono/galactono-kinase, go to the full flat file.
Reaction
Synonyms
2-keto-3-deoxy-D-gluconate kinase, 2-keto-3-deoxygalactonate kinase, 2-keto-3-deoxygluconate kinase, 2-keto-3-deoxyglucononate kinase, HVO_0549, HVO_A0328, KDG kinase, KDGK, KDGK-1, KDGK-2, SSO3195
ECTree
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Cloned
Cloned on EC 2.7.1.178 - 2-dehydro-3-deoxyglucono/galactono-kinase
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produced as inclusion bodies in Escherichia coli when polyhistidine is used as a fusion tag. To reduce inclusion body formation in Escherichia coli, the enzyme is fused with three partners, thioredoxin, glutathione-S-transferase, and N-utilization substance A. With the use of fusion-partners, the solubility of the archaeal protein is remarkably enhanced, and the soluble fraction of the recombinant protein is increased in this order: thioredoxin > glutathione-S-transferase > N-utilization substance A. In the case of recombinant enzyme, the enzyme activity of the Trx-fused protein is 200-fold higher than that of the polyhistidine-fusion protein