2.7.1.178: 2-dehydro-3-deoxyglucono/galactono-kinase

This is an abbreviated version!
For detailed information about 2-dehydro-3-deoxyglucono/galactono-kinase, go to the full flat file.

Reaction

Synonyms

2-keto-3-deoxy-D-gluconate kinase, 2-keto-3-deoxygalactonate kinase, 2-keto-3-deoxygluconate kinase, 2-keto-3-deoxyglucononate kinase, HVO_0549, HVO_A0328, KDG kinase, KDGK, KDGK-1, KDGK-2, SSO3195

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.1 Phosphotransferases with an alcohol group as acceptor

                2.7.1.178 2-dehydro-3-deoxyglucono/galactono-kinase

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Results	in table
8	AA Sequence
4	Cloned(Commentary)
1	Crystallization (Commentary)
3	General Information
4	kcat/KM [mM/s]
4	KM Value [mM]
5	Molecular Weight [Da]
11	Natural Substrates/ Products (Substrates)
53	Organism
17	Pathway
4	pH Optimum
1	pI Value
4	Purification (Commentary)
4	Reaction
9	Reference
19	Substrates and Products (Substrate)
6	Subunits
26	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Temperature Stability [°C]
4	Turnover Number [1/s]

Cloned

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Cloned on EC 2.7.1.178 - 2-dehydro-3-deoxyglucono/galactono-kinase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

expression in Escherichia coli

Saccharolobus solfataricus

Q97U29

672669, 701460, 719488

produced as inclusion bodies in Escherichia coli when polyhistidine is used as a fusion tag. To reduce inclusion body formation in Escherichia coli, the enzyme is fused with three partners, thioredoxin, glutathione-S-transferase, and N-utilization substance A. With the use of fusion-partners, the solubility of the archaeal protein is remarkably enhanced, and the soluble fraction of the recombinant protein is increased in this order: thioredoxin > glutathione-S-transferase > N-utilization substance A. In the case of recombinant enzyme, the enzyme activity of the Trx-fused protein is 200-fold higher than that of the polyhistidine-fusion protein

Saccharolobus solfataricus

Q97U29

694973