2.7.1.190: aminoglycoside 2''-phosphotransferase

This is an abbreviated version!
For detailed information about aminoglycoside 2''-phosphotransferase, go to the full flat file.

Reaction

Synonyms

AAC(6')-APH(2''), AAC(6')-Ie/APH(2'')-Ia, aac6-aph2, aacA-aphD, AME, aminoglycoside (2'') kinase, aminoglycoside 2''-phosphotransferase IVa, aminoglycoside 2''-phosphotransferase type IIIa, aminoglycoside kinase, aminoglycoside phosphotransferase, aminoglycoside-2''-phosphotransferase, aminoglycoside-2''-phosphotransferase type IVa, aminoglycoside-modification enzyme, APH, APH(2''), APH(2'')-Ia, APH(2'')-Ia aminoglycoside resistance enzyme, APH(2'')-Id, Aph(2'')-If, APH(2'')-IIIa, APH(2'')-IVa, aphD, AphSR2, bifunctional 6'-aminoglycoside acetyltransferase/2"-aminoglycoside phosphotransferase, bifunctional AAC/APH, bifunctional aminoglycoside acetyltransferase(6')-Ie/aminoglycoside phosphotransferase(2'')-Ia, bifunctional aminoglycoside acetyltransferase/phosphotransferase, gentamicin kinase, gentamicin phosphotransferase, gentamicin resistance protein

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.1 Phosphotransferases with an alcohol group as acceptor

                2.7.1.190 aminoglycoside 2''-phosphotransferase

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Results	in table
264	AA Sequence
1	Application
7	Cloned(Commentary)
9	Cofactor
7	Crystallization (Commentary)
16	General Information
1	IC50 Value
38	Inhibitors
33	kcat/KM [mM/s]
11	Ki Value [mM]
38	KM Value [mM]
7	Metals/Ions
2	Molecular Weight [Da]
32	Natural Substrates/ Products (Substrates)
20	Organism
24	PDB ID
3	pH Optimum
10	Protein Variants
2	Purification (Commentary)
3	Reaction
17	Reference
70	Substrates and Products (Substrate)
4	Subunits
52	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
36	Turnover Number [1/s]

Cloned

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Cloned on EC 2.7.1.190 - aminoglycoside 2''-phosphotransferase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Enterococcus gallinarum

P96762

734173

expression in Escherichia coli

Enterococcus faecalis

P0A0C2

734051

gene aacA-aphD, synthesis and codon optimization of the gene from Staphylococcus aureus for expression in Nicotiana tabacum from the plastid genome, driven by the psbA gene promoter (PpsbA) from Chlamydomonas reinhardtii, generation of transplastomic lines with the three different aac6-aph2 vectors, recombinant expression in tobacco chloroplasts

Staphylococcus aureus

P0A0C1

758025

gene aphA, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)

Enterococcus sp.

-

760284

gene aphSR2, sequence comparisons, recombinant expression in Escherichia coli strain BL21(DE3), coexpression of aphSR2 gene and genes pkSR1 and pkSR2, encoding serine-threonine protein kinases and localized in one cluster of the Streptomyces rimosus strain ATCC 10970 genome, causing a 2fold increase in resistance to neomycin

Streptomyces rimosus

-

762393

recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3). The catalytic activity of APH(2'')-IVa is independent of the presence of the 6His-tag

Enterococcus casseliflavus

-

760618

recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)

Enterococcus casseliflavus

-

760622