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2.7.1.20: adenosine kinase

This is an abbreviated version!
For detailed information about adenosine kinase, go to the full flat file.

Word Map on EC 2.7.1.20

Reaction

ATP
+
adenosine
=
ADP
+
AMP

Synonyms

adenosine kinase (phosphorylating), ADK, AdK-L, AdK-S, Ado kinase, AdoK, AK, ATP:adenosine 5'-phosphotransferase, CpAK, hADK, kinase, adenosine (phosphorylating), LdAdK, MGG_06270, Rv2202c, Tb927.6.2360, TbAK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.20 adenosine kinase

Purification

Purification on EC 2.7.1.20 - adenosine kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
3600fold purification
-
affinity chromatography
-
ammonium sulfate fractionation and chromatography on DEAE-Sepharose, 20fold purification
-
ammonium sulfate fractionation and gel filtration, 15-20fold purification
-
ammonium sulfate fractionation, affinity chromatography and gel filtration
-
ammonium sulfate fractionation, affinity chromatography, gel filtration and chromatography on DEAE-cellulose
-
ammonium sulfate fractionation, gel filtration ion exchange and affinity chromatography
-
ammonium sulfate precipitation, gel filtration and affinity chromatography, 1600fold purification
-
ammonum sulfate fractionation, chromatography on aminohexyl-Sepharose and gel filtration
-
chromatography on CM-cellulose, chromatography on DEAE-Sephacel and affinity chromatography
-
chromatography on Sephacryl S-200, chromatography on DEAE-cellulose and affinity chromatography, 64fold purification
-
chromatography on Sephadex G-100
Econo-Pac 10DG column chromatography
-
gel filtration and affinity chromatography
gel filtration and affinity chromatography, 870fold purification
-
ion exchange
-
ion exchange and affinity chromatography, partial, 3600fold purification
-
ion exchange, gel filtration and affinity chromatography, 3250fold purification
-
native enzyme 100fold to homogeneity from strain H37Ra
Ni-NTA column chromatography
-
Ni-NTA column chromatography, gel filtration
-
partial
partial, 80fold purification
-
partially, recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
recombinant
recombinant enzyme from Escherichia coli
-
recombinant enzyme from Escherichia coli strain BL21(DE3) by 3 steps of ion exchange chromatography, to homogeneity
-
recombinant enzyme from Escherichia coli to homogeneity
-
recombinant His-tagged isozymes AK-L and AK-S from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged protein from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain M15 by nickel affinity chromatography
-
streptomycin sulfate and ammonium sulfate fractionation, chromatography on DEAE-cellulose and affinity chromatography
-