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2.7.1.21: thymidine kinase

This is an abbreviated version!
For detailed information about thymidine kinase, go to the full flat file.

Word Map on EC 2.7.1.21

Reaction

ATP
+
thymidine
=
ADP
+
dTMP

Synonyms

2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase, beta-thymidine kinase-1, beta-thymidine kinase-2, calmodulin-binding protein, CaM-binding protein, CaMBP, CHV-TK, dThd kinase-1, dThd kinase-2, EC 2.7.1.75, EHV4-TK, FHV-TK, HSV-1 TK, HSV1-TK, kinase, deoxythymidine (phosphorylating), kinase, thymidine (phosphorylating), LMJF_21_1210, mitochondrial thymidine kinase, More, Mt-TK, TdR kinase, ThyB, thymidine kinase, thymidine kinase 1, thymidine kinase 2, thymidine kinase 2, mitochondrial, thymidine kinase I, thymidine kinase-1, thymidine kinase-2, thymidylate kinase, TK, TK-1, TK-2, TK1, TK1a, TK1b, TK2, type 1 thymidine kinase, type II thymidine kinase 1, UL23, VVTK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.21 thymidine kinase

Crystallization

Crystallization on EC 2.7.1.21 - thymidine kinase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
-
Herpes simplex virus
-
complexed with acyclovir and pencyclovir
Herpes simplex virus
-
complexed with an adenine analogue
Herpes simplex virus
-
complexed with deoxythymidine and ganciclovir
Herpes simplex virus
-
hanging drop vapor diffusion method
Herpes simplex virus
-
sitting and hanging drop vapor diffusion method
Herpes simplex virus
-
full length and truncated enzyme in complex with thymidine and ADP, or with thymidine, sulfate, and a bisubstrate analogues, or with TP4A, or with TP5A, hanging drop vapour diffusion method, reservoir solution containing 0.1 M MES, pH 6.5, 1.2 M ammonium sulfate, and 3 or 5% dioxane, dependent on the complex ligand, mixed with enzyme solution containing 5.5 mg/ml protein, 1.0 mM TMP, 2.0 mM ADP, 25 mM HEPES, pH 7.0, 5 mM MgSO4, 5 mM DTT, 100 mM KCl, and 5% glycerol, at equal volumes of 0.001 ml on a glass cover slide, X-ray diffraction structure determination and analysis at 2.0-3.0 A resolution, molecular replacement method
Herpes simplex virus type 4
-
mutant enzyme T163S, hanging drop vapor diffusion method, using 0.1 M sodium citrate (pH 5.6), 20% (v/v) 2-propanol and 17% (w/v) polyethylene glycol 4000
purified recombinant C-terminally truncated enzyme in complex with inhibitor dTTP, hanging drop vapour diffusion method, 7-13 mg/ml protein in solution with 5 mM dTTP mixed with an equal volume of crystallization solution containing 0.1 M Na-citrate, pH 5.6, 20% 2-propanol, and 14-16% PEG 4000, 15°C, X-ray diffraction structure determination and analysis at 2.4-3.5 A resolution
purified recombinant truncated enzyme comprising residues 15-194 of the wild-type enzyme without His-tag, hanging drop vapour diffusion method, 7 mg/ml protein in 5 mM Tris-HCl, pH 7.2, 10 mM NaCl, and 10 mM DTT, mixed at equal volumes with crystallization buffer containing 0.095 mM tri-sodium citrate, pH 5.5, 12% PEG 4000, 10% isopropanol, 23°C, equilibration against a reservoir of 0.5 ml crystallization buffer, 3 days, X-ray diffraction structure determination and analysis at 1.7 A resolution
purified recombinant GST-tagged enzyme complexed with ADP and E-5-(2-bromovinyl)-2'-deoxythymidine, sitting drop vapour diffusion method, 21°C, mixing of 0.001 ml of enzyme and reservoir solution, the enzyme solution contains 8 mg/ml protein, 0.4 mM ADP, 0.2 mM E-5-(2-bromovinyl)-2'-deoxythymidine, and 0.6 mM MgCl2, reservoir solution contains 0.1 M sodium acetate, at either pH 4.5 or 5.0, and 6-11% w/v PEG 20000, X-ray diffraction structure determination and analysis at 3.2 A resolution, molecular replacement method
-
structures of holo forms of the enzyme, in complex with substrate dThd and the non-hydrolysable ATP-analogue P1-(5'-adenosyl)P5-(5'-thymidyl)pentaphosphate
purified recombinant wild-type and selenomethionine-labeled enzyme in complex with inhibitor dTTP, hanging drop vapour diffusion method, 8 mg/ml protein in solution with 5 mM dTTp mixed with an equal volume of reservoir solution containing 19% PEG 2000, 0.2 M LiCl, 5 mM DTT, and 0.1 M MES, pH 6.0, 15°C, X-ray diffraction structure determination and analysis at 2.5 or 3.0 A resolution, respectively
-