2.7.1.21: thymidine kinase
This is an abbreviated version!
For detailed information about thymidine kinase, go to the full flat file.
Word Map on EC 2.7.1.21
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2.7.1.21
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herpes
-
simplex
-
ganciclovir
-
suicide
-
nucleoside
-
hsv-tk
-
acyclovir
-
viruses
-
triphosphate
-
adenovirus
-
prodrugs
-
salvage
-
lymphocyte
-
vaccinia
-
herpesvirus
-
pyrimidine
-
bystander
-
retroviral
-
chloramphenicol
-
positron
-
glioma
-
intratumoral
-
cotransfection
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deoxycytidine
-
varicella-zoster
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cytomegalovirus
-
tomography
-
nude
-
lymphoma
-
dna-mediated
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dtmp
-
deoxynucleoside
-
retrovirus-mediated
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oncolytic
-
adenovirus-mediated
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gene-modified
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deoxyguanosine
-
deoxyribonucleoside
-
synthesis
-
medicine
-
herpetic
-
diagnostics
-
replication-defective
-
neurovirulence
-
3hthymidine
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zidovudine
-
kinase-deficient
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molecular biology
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3'-azido-3'-deoxythymidine
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pseudorabies
-
replication-competent
-
varicella
-
zoster
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5-fluorocytosine
- 2.7.1.21
- herpes
- simplex
- ganciclovir
-
suicide
- nucleoside
-
hsv-tk
- acyclovir
- viruses
- triphosphate
- adenovirus
-
prodrugs
-
salvage
- lymphocyte
- vaccinia
- herpesvirus
- pyrimidine
-
bystander
-
retroviral
- chloramphenicol
-
positron
- glioma
-
intratumoral
-
cotransfection
- deoxycytidine
-
varicella-zoster
- cytomegalovirus
-
tomography
-
nude
- lymphoma
-
dna-mediated
- dtmp
- deoxynucleoside
-
retrovirus-mediated
-
oncolytic
-
adenovirus-mediated
-
gene-modified
- deoxyguanosine
- deoxyribonucleoside
- synthesis
- medicine
-
herpetic
- diagnostics
-
replication-defective
-
neurovirulence
-
3hthymidine
- zidovudine
-
kinase-deficient
- molecular biology
- 3'-azido-3'-deoxythymidine
- pseudorabies
-
replication-competent
- varicella
- zoster
- 5-fluorocytosine
Reaction
Synonyms
2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase, beta-thymidine kinase-1, beta-thymidine kinase-2, calmodulin-binding protein, CaM-binding protein, CaMBP, CHV-TK, dThd kinase-1, dThd kinase-2, EC 2.7.1.75, EHV4-TK, FHV-TK, HSV-1 TK, HSV1-TK, kinase, deoxythymidine (phosphorylating), kinase, thymidine (phosphorylating), LMJF_21_1210, mitochondrial thymidine kinase, More, Mt-TK, TdR kinase, ThyB, thymidine kinase, thymidine kinase 1, thymidine kinase 2, thymidine kinase 2, mitochondrial, thymidine kinase I, thymidine kinase-1, thymidine kinase-2, thymidylate kinase, TK, TK-1, TK-2, TK1, TK1a, TK1b, TK2, type 1 thymidine kinase, type II thymidine kinase 1, UL23, VVTK
ECTree
2.7.1.21 thymidine kinaseAdvanced search results
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results (KCat KM Value
KCat KM Value on EC 2.7.1.21 - thymidine kinase
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kcat/KM VALUE [1/mMs-1] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
5.4
2',3'-dideoxy-2',3'-didehydrothymidine
37°C, pH not specified in the publication
11
2',3'-dideoxythymidine
37°C, pH not specified in the publication
3.8
2'-fluoro-5-methyl-beta-L-arabinofuranosyluracil
37°C, pH not specified in the publication
180
2',3'-dideoxy-3'-azidothymidine
82°C, pH not specified in the publication
450
2',3'-dideoxy-3'-azidothymidine
37°C, pH not specified in the publication
0.0031
3'-azido-3'-deoxythymidine
mutant enzyme T163S, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.0067
3'-azido-3'-deoxythymidine
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.078
3'-azido-3'-deoxythymidine
-
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.35
3'-azido-3'-deoxythymidine
-
mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.44
3'-azido-3'-deoxythymidine
mutant enzyme L124A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.49
3'-azido-3'-deoxythymidine
-
mutant enzyme L134A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.94
3'-azido-3'-deoxythymidine
mutant enzyme M28I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
1.4
3'-azido-3'-deoxythymidine
-
mutant enzyme S182A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
1.7
3'-azido-3'-deoxythymidine
mutant enzyme M28A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
22
3'-azido-3'-deoxythymidine
Q8LDP6, Q9FN47
isoform TK1b, pH and temperature not specified in the publication
48
3'-azido-3'-deoxythymidine
Q8LDP6, Q9FN47
isoform TK1a, pH and temperature not specified in the publication
98
3'-azido-3'-deoxythymidine
-
mutant enzyme S182T, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
740
5-fluoro-2'-deoxyuridine
37°C, pH not specified in the publication
15
ATP
-
full-length enzyme, with 0.05 mM thymidine as cosubstrate, at pH 7.8 and 37°C
40
ATP
-
thymidine kinase domain 2, with 0.05 mM thymidine as cosubstrate, at pH 7.8 and 37°C
0.0019
deoxycytidine
-
mutant enzyme L134A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.048
deoxycytidine
-
mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.0066
deoxyguanosine
-
mutant enzyme S182A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.23
deoxyguanosine
-
mutant enzyme S182T, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
6.8
deoxyuridine
Q8LDP6, Q9FN47
isoform TK1b, pH and temperature not specified in the publication
150
deoxyuridine
Q8LDP6, Q9FN47
isoform TK1a, pH and temperature not specified in the publication
0.0028
thymidine
-
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.0068
thymidine
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.46
thymidine
mutant enzyme T163S, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.51
thymidine
mutant enzyme L124A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
4.2
thymidine
mutant enzyme M28I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
4.5
thymidine
mutant enzyme M28A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
5
thymidine
-
mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
10
thymidine
-
mutant enzyme S182A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
14
thymidine
Q8LDP6, Q9FN47
isoform TK1b, pH and temperature not specified in the publication
15
thymidine
-
mutant enzyme L134A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
750
thymidine
-
mutant enzyme S182T, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
770
thymidine
Q8LDP6, Q9FN47
isoform TK1a, pH and temperature not specified in the publication
