2.7.1.26: riboflavin kinase
This is an abbreviated version!
For detailed information about riboflavin kinase, go to the full flat file.
Word Map on EC 2.7.1.26
-
2.7.1.26
-
mononucleotide
-
ammoniagenes
-
fadss
-
isoalloxazine
-
kyphoplasty
-
flavocoenzyme
-
flavinogenic
-
ribityl
-
davawensis
-
roseoflavin
-
famata
-
ctp-dependent
-
synthesis
-
drug development
- 2.7.1.26
- mononucleotide
- ammoniagenes
-
fadss
- isoalloxazine
-
kyphoplasty
-
flavocoenzyme
-
flavinogenic
-
ribityl
- davawensis
- roseoflavin
- famata
-
ctp-dependent
- synthesis
- drug development
Reaction
Synonyms
AtFMN/FHy, ATP: riboflavin kinase, ATP:riboflavin kinase, bifunctional riboflavin kinase/FMN adenylyltransferase, CaFADS, FAD synthetase, FADS, FK, flavokinase, flavokinase/FAD synthetase, flavokinase/flavin adenine dinucleotide synthetase, FMN adenylyltransferase, FMNAT, HsRFK, kinase, riboflavin, More, RFK, RibC, ribF, riboflavin kinase, riboflavin kinase/FMN adenylyltransferase, riboflavine kinase, RibR
ECTree
2.7.1.26 riboflavin kinaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 2.7.1.26 - riboflavin kinase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2'-dATP + riboflavin
2'-dADP + riboflavin 5'-phosphate
-
-
-
-
?
ATP + 10-(D-allo)flavin
ADP + 10-(D-allo)flavin 5'-phosphate
-
30% of the activity with riboflavin
-
-
?
ATP + 10-(L-arabo)flavin
ADP + 10-(L-arabo)flavin 5'-phosphate
-
25% of the activity with riboflavin
-
-
?
ATP + 2'-deoxyriboflavin
ADP + 2'-deoxyriboflavin 5'-phosphate
-
31% of the activity with riboflavin
-
-
?
ATP + 3-deazariboflavin
ADP + 3-deazariboflavin 5'-phosphate
-
-
-
-
?
ATP + 3-methylriboflavin
ADP + 3-methylriboflavin 5'-phosphate
-
5% of the activity with riboflavin
-
-
?
ATP + 5-deazariboflavin
ADP + 5-deazariboflavin monophosphate
-
-
-
?
ATP + 5-methyl-5-deazariboflavin
ADP + 5-methyl-5-deazariboflavin 5'-phosphate
-
-
-
-
?
ATP + 6,7-dichloro-9-(D-1'-ribityl)isoalloxazine
ADP + 6,7-dichloro-9-(D-1'-ribityl)isoalloxazine phosphate
ATP + 6,7-dimethyl-9-(1'-D-ribityl)-2-iminoisoalloxazine
ADP + 6,7-dimethyl-9-(1'-D-ribityl)-2-iminoisoalloxazine 5'-phosphate
-
18% of the activity with riboflavin
-
-
?
ATP + 6-methylriboflavin
ADP + 6-methylriboflavin 5'-phosphate
-
-
-
-
?
ATP + 7,8-dichlororiboflavin
ADP + 7,8-dichlororiboflavin monophosphate
-
-
-
?
ATP + 8-aminoriboflavin
ADP + 8-aminoriboflavin monophosphate
-
-
-
?
ATP + 8-bromo-8-demethylriboflavin
ADP + 8-bromo-8-demethylriboflavin 5-'phosphate
-
384% of the activity with riboflavin
-
-
?
ATP + 8-chloro-8-demethylriboflavin
ADP + 8-chloro-8-demethylriboflavin 5'-phosphate
-
122.2% of the activity with riboflavin
-
-
?
ATP + 8-dimethylamino-8-demethylriboflavin
ADP + 8-dimethylamino-8-demethylriboflavin 5'-phosphate
ATP + 8-ethoxy-8-demethylriboflavin
ADP + 8-ethoxy-8-demethylriboflavin 5'-phosphate
-
210% of the activity with riboflavin
-
-
?
ATP + 8-fluoro-8-demethylriboflavin
ADP + 8-fluoro-8-demethylriboflavin 5'-phosphate
-
132.2% of the activity with riboflavin
-
-
?
ATP + 8-iodo-8-demethylriboflavin
ADP + 8-iodo-8-demethylriboflavin 5'-phosphate
-
334.7% of the activity with riboflavin
-
-
?
ATP + 8-methoxy-8-demethylriboflavin
ADP + 8-methoxy-8-demethylriboflavin 5'-phosphate
-
114.5% of the activity with riboflavin
-
-
?
ATP + 8-methylamino-8-demethylriboflavin
ADP + 8-methylamino-8-demethylriboflavin 5'-phosphate
-
237.3% of the activity with riboflavin
-
-
?
ATP + 9-azariboflavin
ADP + 9-azariboflavin 5'-phosphate
-
-
-
-
?
CTP + riboflavin
CDP + riboflavin 5'-phosphate
-
50% of the activity with ATP
-
-
?
GTP + riboflavin
GDP + riboflavin 5'-phosphate
-
no activity
-
-
?
ATP + 2-thioriboflavin
ADP + 2-thioriboflavin 5'-phosphate
-
60% of the activity with riboflavin
-
-
?
ATP + 2-thioriboflavin
ADP + 2-thioriboflavin 5'-phosphate
-
30% of the activity with riboflavin
-
-
?
ATP + 5-deazariboflavin
ADP + 5-deazariboflavin 5'-phosphate
-
-
-
-
?
ATP + 5-deazariboflavin
ADP + 5-deazariboflavin 5'-phosphate
-
15% of the activity with riboflavin
-
-
?
ATP + 5-deazariboflavin
ADP + 5-deazariboflavin 5'-phosphate
-
as active as riboflavin
-
-
?
ATP + 6,7-dichloro-9-(D-1'-ribityl)isoalloxazine
ADP + 6,7-dichloro-9-(D-1'-ribityl)isoalloxazine phosphate
-
52% of the activity with riboflavin
-
-
?
ATP + 6,7-dichloro-9-(D-1'-ribityl)isoalloxazine
ADP + 6,7-dichloro-9-(D-1'-ribityl)isoalloxazine phosphate
-
35% of the activity with riboflavin
-
-
?
ATP + 8-demethylriboflavin
ADP + 8-demethylriboflavin 5'-phosphate
-
35% of the activity with riboflavin
-
-
?
ATP + 8-demethylriboflavin
ADP + 8-demethylriboflavin 5'-phosphate
-
110% of the activity with riboflavin
-
-
?
ATP + 8-dimethylamino-8-demethylriboflavin
ADP + 8-dimethylamino-8-demethylriboflavin 5'-phosphate
-
110% of the activity with riboflavin
-
-
?
ATP + 8-dimethylamino-8-demethylriboflavin
ADP + 8-dimethylamino-8-demethylriboflavin 5'-phosphate
-
70% of the activity with riboflavin
-
-
?
ATP + arabitylflavin
ADP + arabitylflavin phosphate
-
14% of the activity with riboflavin
-
-
?
ATP + arabitylflavin
ADP + arabitylflavin phosphate
-
slightly more active than riboflavin
-
-
?
ATP + D-erythroflavin
ADP + D-erythroflavin 5'-phosphate
-
10-(D-erythro)flavin, 33% of the activity with riboflavin
-
-
?
ATP + riboflavin
ADP + FMN
-
specific for the reduced form of riboflavin
-
-
?
ATP + riboflavin
ADP + FMN
ribC is essential for growth of Bacillus subtilis. RibC is not directly involved in the riboflavin regulatory system
-
-
?
ATP + riboflavin
ADP + FMN
-
the flavokinase activity appears to be localized to the N-terminal domain of the protein. RibR specifically interacts in vivo with the nontranslated wild-type leader of the mRNA of the riboflavin biosynthetic operon. In RibR itself, interaction was localized to the carboxy-terminate part of the protein
-
-
?
ATP + riboflavin
ADP + FMN
-
ribC is essential for growth of Bacillus subtilis. RibC is not directly involved in the riboflavin regulatory system
-
-
?
ATP + riboflavin
ADP + FMN
essential enzyme catalyzing the phosphorylation of riboflavin to form FMN, an obligatory step in vitamin B2 utilization and flavin cofactor synthesis
-
-
?
ATP + riboflavin
ADP + FMN
substrate and product binding structure, overview
-
-
ir
ATP + riboflavin
ADP + FMN
-
-
-
-
?
ATP + roseoflavin
ADP + roseoflavin 5'-phosphate
-
187% of the activity with 8-demethylriboflavin
-
-
?
ATP + roseoflavin
ADP + roseoflavin 5'-phosphate
-
81% of the activity with riboflavin
-
-
?
ATP + roseoflavin
ADP + roseoflavin 5'-phosphate
-
90% of the activity with riboflavin
-
-
?
additional information
?
-
ribC wild-type gene product has both flavokinase and flavin adenine dinucleotide synthetase activity
-
-
?
additional information
?
-
-
ribC wild-type gene product has both flavokinase and flavin adenine dinucleotide synthetase activity
-
-
?
additional information
?
-
-
the riboflavin kinase encoding gene ribR of Bacillus subtilis is a part of a 10 kb operon, which is negatively regulated by the yrzC gene product
-
-
?
additional information
?
-
-
ribC wild-type gene product has both flavokinase and flavin adenine dinucleotide synthetase activity
-
-
?
additional information
?
-
bifunctional FAD synthetase exhibiting both the activities of FAD synthetase, EC 2.7.7.2, and riboflavin kinase, EC 2.7.1.26
-
-
?
additional information
?
-
-
bifunctional FAD synthetase exhibiting both the activities of FAD synthetase, EC 2.7.7.2, and riboflavin kinase, EC 2.7.1.26
-
-
?
additional information
?
-
bifunctional FAD synthetase exhibiting both the activities of FAD synthetase, EC 2.7.7.2, and riboflavin kinase, EC 2.7.1.26
-
-
?
additional information
?
-
-
bifunctional FAD synthetase exhibiting both the activities of FAD synthetase, EC 2.7.7.2, and riboflavin kinase, EC 2.7.1.26
-
-
?
additional information
?
-
bifunctional FAD synthetase exhibiting both the activities of FAD synthetase, EC 2.7.7.2, and riboflavin kinase, EC 2.7.1.26
-
-
?
additional information
?
-
-
bifunctional FAD synthetase exhibiting both the activities of FAD synthetase, EC 2.7.7.2, and riboflavin kinase, EC 2.7.1.26
-
-
?
additional information
?
-
the engineered FAD synthetase from Corynebacterium ammoniagenes with deleted N-terminal adenylation domain is a biocatalyst that is stable and efficient for direct and quantitative phosphorylation of riboflavin and riboflavin analogues to their corresponding FMN cofactors at preparative-scale, method evaluation, overview
-
-
?
additional information
?
-
-
the engineered FAD synthetase from Corynebacterium ammoniagenes with deleted N-terminal adenylation domain is a biocatalyst that is stable and efficient for direct and quantitative phosphorylation of riboflavin and riboflavin analogues to their corresponding FMN cofactors at preparative-scale, method evaluation, overview
-
-
?
additional information
?
-
riboflavin kinase activity of the FAD synthetase (EC 2.7.7.2) from Corynebacterium ammoniagenes. Adenine and flavin nucleotide ligands cooperate in their binding to the RFK module
-
-
-
additional information
?
-
-
riboflavin kinase activity of the FAD synthetase (EC 2.7.7.2) from Corynebacterium ammoniagenes. Adenine and flavin nucleotide ligands cooperate in their binding to the RFK module
-
-
-
additional information
?
-
substrate binding structure analysis using crystal structures of substrate-bound RFK. Binding of HsRFK substrates is the kinetically preferred process compared to product inhibition, kinetic modeling, overview
-
-
-
additional information
?
-
-
substrate binding structure analysis using crystal structures of substrate-bound RFK. Binding of HsRFK substrates is the kinetically preferred process compared to product inhibition, kinetic modeling, overview
-
-
-
additional information
?
-
-
no phosphorylation of isoriboflavin, galactoflavin, dulcitylflavin, sorbitylflavin
-
-
?
additional information
?
-
the bifunctional flavokinase/flavin adenine dinucleotide synthetase produces inactive flavin cofactors and is not involved in resistance to the antibiotic roseoflavin
-
-
?
additional information
?
-
-
the bifunctional flavokinase/flavin adenine dinucleotide synthetase produces inactive flavin cofactors and is not involved in resistance to the antibiotic roseoflavin
-
-
?
additional information
?
-
bifunctional FAD synthetase exhibiting both the activities of FAD synthetase, EC 2.7.7.2, and riboflavin kinase, EC 2.7.1.26
-
-
?
additional information
?
-
the flavin motif is involved in flavin ligand binding, role of active site residues in the catalytic mechanism, overview. The isoalloxazine ring is sandwiched between the indole ring of Trp184 and the planar guanidinium group of Arg189, while the hydrophilic pyrimidine ring forms two specific hydrogen bonds between its C4 carbonyl and the main chain amide of Asp181, and between its N3 amide and the side chain of Asp181, respectively. Residues Asn62, Asp66, Asp168, and Arg297 interact either with ATP phosphate groups, or to coordinate the catalytic Mg2+ ion either directly or indirectly through water molecules. Arg297 might be involved in the interaction with the phosphate groups of both substrates, and helps in their positioning for the nucleophilic attack in the adenylyltransfer reaction
-
-
?
