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2.7.1.29: glycerone kinase

This is an abbreviated version!
For detailed information about glycerone kinase, go to the full flat file.

Word Map on EC 2.7.1.29

Reaction

ATP
+
Glycerone
=
ADP
 +
glycerone phosphate

Synonyms

acetol kinase, ATP-dependent dihydroxyacetone kinase, dAK, DHA kinase, DhaK, DhaK-2, DhaKI, DhaL, dihydroxyacetone kinase, dihydroxyacetone kinase I, FMN cyclase/dha kinase, glycerone kinase, kinase, acetol (phosphorylating), More, PEP-dependent Dha kinase, phosphoenolpyruvate carbohydrate phosphotransferase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.29 glycerone kinase

Crystallization

Crystallization on EC 2.7.1.29 - glycerone kinase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structure of enzyme complex with an ATP analogue and dihydroxyacetone at 2.5 A resolution
apoenzyme, from solution containing 80 mM sodium acetate, pH 5.0, 160 mM ammonium sulfate, 17% w/v PEG 4000, 15% w/v methylpentanediol, hanging drop vapour diffusion method, apoenzyme-crystals are soaked in 2 mM glyceraldehyde or 5 mM dihydroxyacetone phosphate and flash frozen at -168°C, X-ray diffraction structure determination and analysis at 2.0 and 1.9 A resolution, respectively
crystal structure of the nucleotide-binding subunit DhaL
DhaK and DhaK-dihydroxacetone complex are crystallized from 80 mM sodium acetate, pH 5.0, 160 mM ammonium sulfate, 17% polyethylene glycol 4000, 15% 2-methyl-2,4-pentanediol using hanging drop vapor diffusion, crystals diffract to 1.75 A resolution
DhaK-DhaL complex bound to ADP anddihydroxyacetone, hanging drop vapor diffusion method, using 0.1 M HEPES pH 7.5, 3.5 M sodium formate or 0.1 M sodium citrate pH 5.6, 20% (w/v)PEG 8000, at 19°C