2.7.1.3: ketohexokinase

This is an abbreviated version!
For detailed information about ketohexokinase, go to the full flat file.

Reaction

Synonyms

ATP:D-fructose 1-phosphotransferase, fructokinase, ketohexokinase, ketohexokinase (phosphorylating), KHK, Khk-A, Khk-C

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.1 Phosphotransferases with an alcohol group as acceptor

                2.7.1.3 ketohexokinase

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Results	in table
47	AA Sequence
3	Application
1	CAS Registry Number
6	Cloned(Commentary)
4	Crystallization (Commentary)
79	Disease/ Diagnostics
11	General Information
2	General Stability
63	IC50 Value
99	Inhibitors
2	Ki Value [mM]
55	KM Value [mM]
26	Metals/Ions
9	Molecular Weight [Da]
17	Natural Substrates/ Products (Substrates)
36	Organism
4	Pathway
51	PDB ID
5	pH Optimum
2	pH Range
3	pI Value
3	Protein Variants
8	Purification (Commentary)
2	Reaction
1	Reaction Type
35	Reference
57	Source Tissue
9	Specific Activity [micromol/min/mg]
2	Storage Stability
70	Substrates and Products (Substrate)
4	Subunits
29	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Temperature Range [°C]
4	Temperature Stability [°C]
12	Turnover Number [1/s]

Temperature Stability

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Temperature Stability on EC 2.7.1.3 - ketohexokinase

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

25

Haloarcula vallismortis

-

25°C, 2 h, totally inactivated in 0.1 M KCl/50 mM Tris-HCl (pH 9.0). Stabilizing effect of KCl increases with increasing KCl concentration between 0.1 and 2.5 M (ranging from 30 to 98% of the total). Addition of 50 mM phosphate aids in stabilization of ketohexokinase activity in 0.1 M KCI for about 24 h. Although it is inhibitory for expression of ketohexokinase activity, (NH4)2SO4 stabilizes

727726

37

Homo sapiens

-

C-A43T rapidly loss in activity in absence of glycerol

641285

55

Homo sapiens

-

30 min ketohexokinase-A little loss in activity, ketohexokinase-C completely inactivated

641285

75

Haloarcula vallismortis

-

30 min, 5% loss of activity

727726