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2.7.1.30: glycerol kinase

This is an abbreviated version!
For detailed information about glycerol kinase, go to the full flat file.

Word Map on EC 2.7.1.30

Reaction

ATP
+
glycerol
=
ADP
+
sn-glycerol 3-phosphate

Synonyms

AFUB_068560, ASTP , ATP-stimulated glucocorticoid-receptor translocation promoter , ATP: glycerol 3-phosphotransferase, ATP: glycerol-3-phosphotransferase, ATP:glycerol 3-phosphotransferase, ATP:glycerol 3-phosphotransferase , ATP:glycerol-3-phosphotransferase, GK, GK1, GK2, GK3, GK4, GK5, glcA, GLPK, glyceric kinase, glycerokinase, glycerol kinase, glycerol kinase 2, glycerol kinase 5, GLYK, GUT1, GYK, kinase, glycerol (phosphorylating), KpGlpK, More, slr1672, TbgGK, Tk-GK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.30 glycerol kinase

General Stability

General Stability on EC 2.7.1.30 - glycerol kinase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
(NH4)2SO4 stabilizes
-
dialysis, against 0.01 M phosphate buffer, at 2°C, pH 6.7, 50% loss of activity without glycerol, stable in presence of 0.01 M glycerol
Candida mycoderma
-
ethanediol stabilizes
Candida mycoderma
-
freezing without glycerol inactivates
-
glycerol affords considerable stabilization at the unfavorable pH values
-
glycerol, 0.01 M, EDTA, 0.001 M and 0.001 M 2-mercaptoethanol prevent inactivation during purification
-
normal and mutant enzyme stabilized against heat inactivation by glycerol, but not by fructose 1,6-bisphosphate
-
pigeon liver enzyme is sensitive to extreme dilution but can be stabilized by addition of 0.01% bovine serum albumin
-
the enzyme in crude extracts is stable to freezing and thawing, while more purified preparations are inactivated