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2.7.1.32: choline kinase

This is an abbreviated version!
For detailed information about choline kinase, go to the full flat file.

Word Map on EC 2.7.1.32

Reaction

ATP
+
choline
=
ADP
+
phosphocholine

Synonyms

ATP/choline phosphotransferase, ATP:choline phosphotransferase, CEK1, CEK2, CEK3, chbA, CHETK-alpha, CHK, chk-alpha, Chka, CHKA2, ChKalpha, ChoK, ChoK alpha, ChoKalpha, CHOKalpha1, ChoKalpha2, ChoKbeta1, choline kinase, choline kinase alpa, choline kinase alpha, choline kinase alpha1, choline kinase alpha2, choline kinase beta, choline kinase beta1, choline kinase isoform alpha2, choline kinase-alpha, choline phosphokinase, choline-ethanolamine kinase, choline/ethanolamine kinase, choline/ethanolamine kinase 1, choline/ethanolamine kinase 2, choline/ethanolamine kinase 3, CK, CK-alpha, CK-alpha1, CK-alpha1/beta, CK-alpha2, CK-beta, CK1, CK2, CK3, CKA-2, CKalpha, CKalpha1, CKalpha2, ckb, CKbeta, CKbeta1, CKI1, CKI1-encoded choline kinase, CKII, ER-localized Cho kinase, EtnK, hChoK, kinase, choline (phosphorylating), LicA, LinfC/EK35, More, PfChoK, PfCK, protein LicA, putative choline kinase, sChoK, TbC/EK2

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.32 choline kinase

Engineering

Engineering on EC 2.7.1.32 - choline kinase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C252S
-
slightly increased turnover
D255A
D255E
D255N
D301A
D301E
D301N
D313A
-
low expression level
E125A
E125D
E125Q
E134A
-
slightly increased turnover
E151A
-
slightly increased turnover
E303A
E303D
E303N
-
small reduction of kcat
E303Q
-
reduced activity
E320A
H181A
-
low expression level
H253A
N254A
-
similar initial activity like wild type enzyme, but time instability
N260A
N308 A
-
small reduction of kcat
N308A
-
reduced activity
N308D
N308Q
N354A
-
strongly reduced activity, only minor effect on Km for both substrates
R111A
R149A
R149K
-
slightly increased turnover
S85T
-
reduced activity
S86T
-
moderate reduction of the catalytic efficiency
W387A
D255A
-
complete loss of activity
D306A
E125A
E255A
-
almost complete loss of activity
E303A
-
10fold decrease in activity
E320A
-
26fold higher Km for ATP
F352A
the mutant exhibits a higher turnover number compared to the wild type enzyme
F352L
the mutant exhibits a higher turnover number compared to the wild type enzyme
L401A
the mutant of isoform ChoKalpha1 exhibts a lower turnover number compared to the wild type enzyme
L401F
the mutant of isoform ChoKalpha1 exhibts a lower turnover number compared to the wild type enzyme
N255A
-
complete loss of activity
P59A/P60A
site-directed mutagenesis
P61A/P62A
site-directed mutagenesis
P72A/P73A
site-directed mutagenesis
R111A
-
2-25% catalytic efficiency compared to the wild type enzyme
S39D/S40D
the choline kinase beta phosphorylation mimic behaves kinetically like the wild type enzyme
S86A
-
2-25% catalytic efficiency compared to the wild type enzyme
W387A
-
2-25% catalytic efficiency compared to the wild type enzyme
Y197/333F
the mutant shows reduced activity in the presence of epidermal growth factor receptor compared to the wild type enzyme
Y197F
the mutant shows reduced activity in the presence of epidermal growth factor receptor compared to the wild type enzyme
Y333F
the mutant shows reduced activity in the presence of epidermal growth factor receptor compared to the wild type enzyme
S25A
-
mutation results in 32% decrease of phosphatidylcholine synthesis compared to the wild type enzyme
S25D
-
mutation results in 14% increase of phosphatidylcholine synthesis compared to the wild type enzyme
S30A
-
specific activity reduced by 44%, phosphorylation reduced by 70%
S30A/S85A
-
specific activity reduced by 60%, phosphorylation reduced by 83%
S85A
-
specific activity reduced by 8%, phosphorylation reduced by 17%
S25A
-
mutation results in 32% decrease of phosphatidylcholine synthesis compared to the wild type enzyme
-
S25D
-
mutation results in 14% increase of phosphatidylcholine synthesis compared to the wild type enzyme
-
D167A
the mutant shows about 14% activity compared to the wild type enzyme
T29A
the mutant shows about 10% activity compared to the wild type enzyme
T29S
the mutant shows slightly increased activity compared to the wild type enzyme
additional information