2.7.1.32: choline kinase
This is an abbreviated version!
For detailed information about choline kinase, go to the full flat file.
Word Map on EC 2.7.1.32
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2.7.1.32
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phosphatidylcholine
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phospholipid
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phosphocholine
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cytidylyltransferase
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cdp-choline
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phosphorylcholine
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phosphatidylethanolamine
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ctp:phosphocholine
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cholinephosphotransferase
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kennedy
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cholinephosphate
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glycerophosphocholine
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phosphatidate
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choline-containing
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hemicholinium-3
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phosphoethanolamine
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bispyridinium
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3hcholine
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2.7.7.15
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ptdcho
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diagnostics
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medicine
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1,2-diacylglycerol
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pcyt1a
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drug development
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cdp-ethanolamine
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biotechnology
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2.7.8.2
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phosphatidylcholine-specific
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methyl-3hcholine
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methyl-14ccholine
- 2.7.1.32
- phosphatidylcholine
- phospholipid
- phosphocholine
- cytidylyltransferase
- cdp-choline
- phosphorylcholine
- phosphatidylethanolamine
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ctp:phosphocholine
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cholinephosphotransferase
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kennedy
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cholinephosphate
- glycerophosphocholine
- phosphatidate
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choline-containing
- hemicholinium-3
- phosphoethanolamine
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bispyridinium
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3hcholine
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2.7.7.15
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ptdcho
- diagnostics
- medicine
- 1,2-diacylglycerol
- pcyt1a
- drug development
- cdp-ethanolamine
- biotechnology
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2.7.8.2
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phosphatidylcholine-specific
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methyl-3hcholine
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methyl-14ccholine
Reaction
Synonyms
ATP/choline phosphotransferase, ATP:choline phosphotransferase, CEK1, CEK2, CEK3, chbA, CHETK-alpha, CHK, chk-alpha, Chka, CHKA2, ChKalpha, ChoK, ChoK alpha, ChoKalpha, CHOKalpha1, ChoKalpha2, ChoKbeta1, choline kinase, choline kinase alpa, choline kinase alpha, choline kinase alpha1, choline kinase alpha2, choline kinase beta, choline kinase beta1, choline kinase isoform alpha2, choline kinase-alpha, choline phosphokinase, choline-ethanolamine kinase, choline/ethanolamine kinase, choline/ethanolamine kinase 1, choline/ethanolamine kinase 2, choline/ethanolamine kinase 3, CK, CK-alpha, CK-alpha1, CK-alpha1/beta, CK-alpha2, CK-beta, CK1, CK2, CK3, CKA-2, CKalpha, CKalpha1, CKalpha2, ckb, CKbeta, CKbeta1, CKI1, CKI1-encoded choline kinase, CKII, ER-localized Cho kinase, EtnK, hChoK, kinase, choline (phosphorylating), LicA, LinfC/EK35, More, PfChoK, PfCK, protein LicA, putative choline kinase, sChoK, TbC/EK2
ECTree
2.7.1.32 choline kinaseAdvanced search results
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results (Specific Activity
Specific Activity on EC 2.7.1.32 - choline kinase
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SPECIFIC ACTIVITY [µmol/min/mg] 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
0.000139
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skeletal muscle, hindlimbs of +/+ CK-alpha mice, supernatant of tissue homogenates are incubated in volume of 0.100 ml of reaction buffer at 37°C for 30 min. The reaction product, phosphocholine, is separated using an AG1-X8 column.To determine the activity of each CK isoform, supernatant fractions are treated with an antisera raised against GST (control), GST-CK-alpha or GST-CK-beta fusion proteins combined with protein A-Sepharose overnight at 4°C, and the supernatant is used.
0.000484
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in 40 mM Tris/HCl with 10 mM MgCl2, 2 mM ATP and 0.25 mM choline, pH 7.4, at 37°C
10.67
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2 microgram of purified protein is incubated with a reaction mixture of 100 mM MOPS (pH 7.8), 6 mM MgCl2 and 5 mM ATP, with either 2 mM ethanolamine and 200 nanoCi of [3H]ethanolamine or 2 mM choline and 200 nanoCi of (3H)choline at 37°C for 20 min and quenched by boiling at 100°C for 5 min. Substrates and products are separated by high-performance TLC using silica 60 plates with methanol/0.6% NaCl/ammonium hydroxide as solvent. Radiolabelled species are detected by fluorography at -70°C.
40.4
additional information
additional information
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exponentially growing cells: 484.04 pmol/mg/min (activity is determined after homogenizing a cell pellet in Tris/HCl buffer. Homogenisate of exponentially growing and confluent cells is centrifugated. Assay mixture containing 40 mM Tris/HCl with unlabelled carrier of 0.25 mM choline, (methyl-3H)choline (37 kBq/sample), 2 mM ATP and 10 mM MgCl2 were added to homogenate protein. After incubation, reaction is stopped by addition of ice-cold mixture of methanol and chloroform and lipid-metabolite extraction is then carried out. Protein content is set for each sample and total radioactivity is determined. Separation of (methyl-3H)-phosphocholine and (methyl-3H)-choline is carried out using ion exchange chromatography)
additional information
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Genes significantly down-regulated by choline kinase: BCL2-interacting killer (apoptosis-inducing), stabilization of the calcium channel, cyclin G2. Inhibition of cell proliferation, T-cell surface protein tactile, cyclin-dependent kinase inhibitor 1A (p21, Cip1), cyclin-dependent kinase inhibitor 1B (p27, Kip1), dual-specificity-(Y)-tyrosine phosphor regulated kinase, Mad signalling (altered response to quimiotherapy), opioid receptor, mu 1 (negative regulat of cell proliferation), retinoblastoma-like 1 (p107) (Rb associated protein), transforming growth factor, beta receptor I, Toll like receptor (antitumoral immunity), immune escape of human cancer, Visinin like 1
additional information
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increase in mRNA levels results in an increase in protein expression and ChoK enzymatic activity
additional information
62.2 micromol/min/mg for ethanolamine
additional information
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The specific activity of choline kinase in wild type zinc-depleted cells is 2.7fold greater when compared with the activity from cells grown with 1.5 microM ZnSO4. Choline kinase activity is measured in a reaction mixture that contains 67 mM glycine-NaOH, 5 mM (methyl-14C)choline, 5 mM ATP, 10mM MgSO4 and 1.3 mM dithiothreitol. The radiolabeled product phosphocholine, is separated from the substrate by precipitation of choline as a reineckate salt.
